生长素对百合呈色合成调控的研究  

The Regulation of Auxin on Anthocyanin Biosynthesis in Lilium Brownii

作　　者：孙程程 徐华[1,2] 李晓辉 曹雨薇 SUN Chengcheng;XU Hua;LI Xiao hui;CAO Yu wei(College of Life Sciences,Gannan Normal University,Ganzhou 341000,China;Key Laboratory of Nanling Plant Resources Protection and Utilization,Gannan Normal University,Ganzhou 341000,China;Information and Educational Technology Center,Gannan Normal University,Ganzhou 341000,China)

机构地区：[1]赣南师范大学生命科学学院,江西赣州341000 [2]赣南师范大学赣州市南岭植物资源保护与利用重点实验室,江西赣州341000 [3]赣南师范大学信息与教育技术中心,江西赣州341000

出　　处：《赣南师范大学学报》2024年第3期56-60,共5页Journal of Gannan Normal University

基　　金：江西省自然科学基金(20232BAB215044);江西省教育厅科学计划项目(GJJ2201232);赣南师范大学博士科研启动基金(BSJJ202126)。

摘　　要：生长素作为植物生长调节激素,具有参与花色合成的功能.本研究通过对百合鳞片喷施不同浓度生长素及其抑制剂,发现抑制剂处理后提前呈色,而200 mg/L IAA处理花青素苷含量最高,300 mg/L IAA花青素苷含量最低.并利用实时荧光定量技术(Real-time quantitative PCR,RT-qPCR)对花青素苷合成和调控相关基因的表达进行检测,发现抑制剂NPA和TIBA处理后相关基因的表达均表现为显著上调.通过对生长素调控百合鳞片花青素苷合成的研究,初步探究生长素对百合呈色合成的调控作用,以期丰富激素对单子叶植物百合的呈色调控网络.Auxin,as a plant growth regulating hormone,has the function of participating in anthocyanin biosynthesis.Here,lily scales were sprayed different concentrations of auxin and its inhibitors,and it was found that the inhibitor resulted in early coloration,while the 200 mg/L IAA had the highest anthocyanin content and the 300 mg/L IAA had the lowest.Meanwhile,the expression of genes related to anthocyanin biosynthesis and regulation was significantly upregulated under the treatment of inhibitors NPA and TIBA by RT-qPCR.With the study of auxin regulation on anthocyanin biosynthesis of lily scales,it preliminarily explores the regulation of auxin on lily pigmentation,to enrich the color regulatory network of hormones on monocotyledons lily.

关 键 词：龙牙百合 生长素 花青素苷 转录调控 

分 类 号：S68[农业科学—观赏园艺]