Drug-loading ZIF-8 for modification of microporous bone scaffold to promote vascularized bone regeneration  

作　　者：Xin Zhang Junyu Chen Xiang Pei Linxin Yang Liang Wang Luona Chen Guangmei Yang Xibo Pei Qianbing Wan Jian Wang 

机构地区：[1]State Key Laboratory of Oral Diseases,National Clinical Research Center for Oral Diseases,Chengdu 610041,China [2]Department of Prosthodontics,West China Hospital of Stomatology,Sichuan University,Chengdu 610041,China

出　　处：《Chinese Chemical Letters》2024年第6期358-364,共7页中国化学快报（英文版）

基　　金：supported by the National Natural Science Foundation of China(Nos.82201128,82271034);Special Funding for Post-doctoral Research Projects in Sichuan Province(No.TB2022045);Sichuan Province Science and Technology Plan Projects(No.23NSFSC1723);China Postdoctoral Science Foundation(No.2022M722250);Research and Development Program(West China Hospital of Stomatology Sichuan University)(Nos.RD-02–2022012,RD-03–202107)。

摘　　要：Surface modification of microporous bone scaffolds using nanoparticles has been broadly studied in bone tissue engineering.Aiming at improving vascularized bone regeneration(VBR),zeolitic imidazolate framework-8(ZIF-8)was encapsulated with dimethyloxallyl glycine(DMOG)and the drug-carrying nanoparticles(D@Z)could be uniformly coated onto the surface of the bone scaffold.The osteogenic and angiogenic actions of D@Z are closely correlated with the amount of slowly released DMOG,and in general,exhibited a favorable association.Then,the D7.5@Z group,which showed the greatest capacity to induce in vitro osteogenesis-angiogenesis coupling,was utilized for surface modification of the bone scaffold.Biological processes including phosphate-containing compound metabolic process,cell differentiation,cell proliferation and cell motility might contribute to enhanced ability to induce VBR by the coated scaffold and signaling pathways such as Rap1,Ras,phosphatidylinositol 3-kinase/protein kinase B(PI3K-AKT)and vascular endothelial growth factor(VEGF)signaling pathways participated in these processes.Finally,as depicted by in vitro real time-polymerase chain reaction(RT-PCR),Western blot(WB)and in vivo cranial bone defect model,the microporous scaffold coated with nano-D7.5@Z greatly promoted VBR.To conclude,nano-D@Z has significant promise for practical application in modification of microporous bone scaffolds to enhance VBR,and DMOG loading quantity has a beneficial influence on D@Z to improve osteogenesis-angiogenesis coupling.

关 键 词：Drug-loading nanoparticles Surface coating Microporous bone scaffold VASCULARIZATION bone regeneration 

分 类 号：TQ460.1[化学工程—制药化工] TB383.1[一般工业技术—材料科学与工程] R318.08[医药卫生—生物医学工程]