A mitochondria-targeted H_(2)S-activatable fluorogenic probe for tracking hepatic ischemia-reperfusion injury  

作　　者：Bin Fang Jiaqi Yang Limin Wang Haoqin Li Jiaying Guo Jiaxin Zhang Qingyuan Guo Bo Peng Kedi Liu Miaomiao Xi Hua Bai Li Fu Lin Li 

机构地区：[1]School of Materials Science and Engineering,Northwestern Polytechnical University,Xi’an 710072,China [2]Frontiers Science Center for Flexible Electronics,Xi’an Institute of Flexible Electronics(IFE)and Xi’an Institute of Biomedical Materials&Engineering,Northwestern Polytechnical University,Xi’an 710072,China [3]Key laboratory of Flexible Electronics of Zhejiang Province,Ningbo Institute of Northwestern Polytechnical University,Ningbo 315103,China [4]The Institute of Flexible Electronics(IFE,Future Technologies),Xiamen University,Xiamen 361005,China [5]Wuhan National Laboratory for Optoelectronics-Advanced Biomedical Imaging Facility,Huazhong University of Science and Technology,Wuhan 430074,China [6]TANK Medicinal Biology Institute of Xi’an,Xi’an 710032,China

出　　处：《Chinese Chemical Letters》2024年第6期370-375,共6页中国化学快报（英文版）

基　　金：financially supported by the National Natural Science Foundation of China(Nos.22077101,22004099);the Joint Research Funds of Department of Science&Technology of Shaanxi Province and Northwestern Polytechnical University(Nos.2020GXLH-Z-008,2020GXLH-Z-021,2020GXLH-Z-023);Natural Science Foundation of Shaanxi Province(No.2022JM-130);The Natural Science Foundation of Ningbo(Nos.202003N4049,202003N4065);the Open Project Program of Wuhan National Laboratory for Optoelectronics(Nos.2020WNLOKF023,2022WNLOKF009);Innovation Foundation for Doctor Dissertation of Northwestern Polytechnical University(No.CX2022034);Innovation Capability Support Program of Shaanxi(No.2023-CX-PT-23)。

摘　　要：Hepatic ischemia-reperfusion injury(HIRI)is the cause of postoperative hepatic dysfunction and failure,and even death.As an important biological effector molecule,hydrogen sulfide(H_(2)S)of mitochondria as a gasotransmitter that is usually used to protect against acute HIRI injury.However,the exact relationship between HIRI and mitochondrial H_(2)S remains tangled due to the lack of an effective analytical method.Herein,we have fabricated a mitochondria-targeted H_(2)S-activatable fluorogenic probe(Mito-GW)to explore the stability of mitochondrial H_(2)S and track the changes of mitochondrial H_(2)S during the HIRI.By virtue of pyridinium electropositivity and its amphiphilicity,Mito-GW could accumulate in mitochondria.It goes through an analyte-prompted immolation when reacts with H_(2)S,resulting in the releasing of the fluorophore(GW).Therefore,the extent of Mito-GW conversion to GW can be used to evaluate the changes of mitochondrial H_(2)S level in living cells and tissues.As proof-of-principle,we have used MitoGW to demonstrate the mitochondria H_(2)S-levels increase and then decrease during HIRI in vitro and in vivo.Our research highlights the tremendous potential of Mito-GW as a mitochondrial H_(2)S fluorogenic probe in elucidating the pathogenesis of HIRI,providing a powerful tool for promoting future research on hepatology.

关 键 词：Mitochondria-targeted Bioimaging H2S-activatable Fluorogenic probe Hepatic ischemia-reperfusion injury(HIRI) 

分 类 号：O657.3[理学—分析化学] R575[理学—化学]