4-Nitrocatechol as a novel matrix for low-molecular-weight compounds in situ detection and imaging in biological tissues by MALDI-MSI  

作　　者：Hualei Xu Manman Han Haiqiang Liu Liang Qin Lulu Chen Hao Hu Ran Wu Chenyu Yang Hua Guo Jinrong Li Jinxiang Fu Qichen Hao Yijun Zhou Jinchao Feng Xiaodong Wang 

机构地区：[1]Key Laboratory of Mass Spectrometry Imaging and Metabolomics(Minzu University of China),State Ethnic Affairs Commission,Beijing 100081,China [2]College of Life and Environmental Sciences,Centre for Imaging&Systems Biology,Minzu University of China,Beijing 100081,China

出　　处：《Chinese Chemical Letters》2024年第6期486-490,共5页中国化学快报（英文版）

基　　金：supported by the National Natural Science Foundation of China(Nos.31770384 and 21605164);the Youth Academic Team Project of MUC(No.10301-02200301);the Huayi Technology Innovation Center for Research Resources(No.HTIC P01RR2017001A);the Key Laboratory Construction Funds of State Ethnic Affairs Commission of China(No.10301-02200303)。

摘　　要：Low-molecular-weight(LMW)compounds are ubiquitous in living organisms and play essential roles in biological processes.The direct analysis of LMW compounds in biological tissues by matrix-assisted laser desorption/ionization mass spectrometry imaging(MALDI-MSI)could provide a more comprehensive understanding of their essential functions.Here,we evaluated 4-nitrocatechol(4-NC)as a novel positive-ion matrix for enhancing in situ detection and imaging of LMW compounds from the rat liver,brain,and germinating Chinese-yew seed by MALDI-MS.Our results showed that the 4-NC possessed remarkable features,including strong ultraviolet absorption,uniform matrix crystal,excellent chemical stability,and fewer matrix-related background peaks.The use of 4-NC led to the successful detection of 232,218,and193 LMW compounds from the three abovementioned tissue sections,respectively.Also,the use of 4-NC improved the imaging quality of LMW compounds in tissue sections through MALDI-MSI and has the potential as a matrix for MALDI tissue imaging of LMW compounds.

关 键 词：MALDI-MSI 4-Nitrocatechol LMW compounds In situ detection Tissue imaging 

分 类 号：O657.63[理学—分析化学] Q503[理学—化学]