Octopus-like DNA nanostructure coupled with graphene oxide enhanced fluorescence anisotropy for hepatitis B virus DNA detection  

作　　者：Jia-Li Xie Tian-Jin Xie Yu-Jie Luo Kai Mao Cheng-Zhi Huang Yuan-Fang Li Shu-Jun Zhen 

机构地区：[1]Key Laboratory of Luminescence Analysis and Molecular Sensing(Southwest University),Ministry of Education,College of Chemistry and Chemical Engineering,Southwest University,Chongqing 400715,China [2]Key Laboratory of Luminescent and Real-Time Analytical Chemistry(Southwest University),Chongqing Science and Technology Bureau,College of Pharmaceutical Sciences,Southwest University,Chongqing 400715,China

出　　处：《Chinese Chemical Letters》2024年第6期531-535,共5页中国化学快报（英文版）

基　　金：supported by the National Natural Science Foundation of China(Nos.21974109,22322409);the Natural Science Foundation of Chongqing(No.CSTB2022NSCQ-MSX1662);the Fundamental Research Funds for the Central Universities(No.XDJK2019TY003)。

摘　　要：Fluorescence Anisotropy(FA)is an effective biochemical detection method based on molecular rotations.Graphene oxide(GO)has been extensively used as an FA amplifier.However,the enhancement of FA by GO alone is limited and the strong scattering of GO will easily make the measurement of FA inaccurate.In order to address these problems,an octopus-like DNA nanostructure(ODN)was designed and coupled with GO to enhance the FA together in this work.By mimicking the multi-clawed structure of the octopus,the ODN can be adsorbed on GO tightly,which not only could improve the sensitivity because of the double FA enhancement abilities of GO and ODN,but also could improve the specificity due to the decrease of the nonspecific interaction in complex samples.Furthermore,ODN could maintain a certain distance between the fluorophore and GO to reduce the fluorescence quenching efficiency of GO,which could improve the accuracy.This method has been applied for the detection of hepatitis B virus DNA(HBV-DNA)in a range of 1-50 nmol/L and the limit of detection(LOD)was 330 pmol/L.In addition,the proposed method has been successfully utilized to detect HBV-DNA in human serum,indicating that this method has a great practical application prospect.

关 键 词：Fluorescence anisotropy Graphene oxide(GO) Octopus-like DNA nanostructure HBV-DNA 

分 类 号：O657.3[理学—分析化学] R313[理学—化学]