Au-Fe_(3)O_(4) dumbbell-like nanoparticles based lateral flow immunoassay for colorimetric and photothermal dual-mode detection of SARS-CoV-2 spike protein  

作　　者：Gengchen Guo Tianyu Zhao Ruichang Sun Mingzhe Song Hongyu Liu Sen Wang Jingwen Li Jingbin Zeng 

机构地区：[1]College of Chemistry and Chemical Engineering and State Key Laboratory of heavy oil processing,China University of Petroleum(East China),Qingdao 266580,China [2]Huangdao Customs of the People’s Republic of China,Qingdao 266580,China

出　　处：《Chinese Chemical Letters》2024年第6期536-540,共5页中国化学快报（英文版）

基　　金：supported by the Natural Scientific Foundation of Shandong(Nos.ZR2023MC039,ZR2022JQ07);the National Natural Science Foundation of China(No.21876206);the Fundamental Research Funds for the Central Universities(No.21CX06014A);the Taishan Scholarship of Shandong Province(No.tsqn202211080)。

摘　　要：Lateral flow immunoassay(LFIA)has become popular in laboratories,at-home testing,and medical diagnostics due to its minimal cost and user-friendliness.Nevertheless,conventional test strips based on colloidal gold can only obtain qualitative or semi-quantitative results with low sensitivity.In this work,AuFe_(3)O_(4) dumbbell-like nanoparticles were synthesized and used as the LFIA labelling marker for highly sensitive colorimetric-photothermal dual-mode detection of SARS-CoV-2 spike(S)protein.The unique dumbbell structure of Au-Fe_(3)O_(4) NPs makes it possible to combine the best features of both Au NPs and Fe_(3)O_(4) NPs.The increased surface area of these NPs enhances their LSPR effect and photothermal effect,which achieves signal amplification to increase sensitivity.The Au-Fe_(3)O_(4) NPs modified with S protein antibody could identify S protein in samples,which were recognized and accumulated on T-line by another antibody,generating color band for qualitative colorimetric detection.The T-line was irradiated by laser to obtain temperature change for quantitative detection of photothermal.In optimized conditions,the detection limit was 1.22 pg/m L,three orders of magnitude more sensitive than colorimetric detection.Finally,the approach was performed on SARS-CoV-2 pseudovirus samples and outperformed traditional colloidal gold strips.This LFIA platform exhibits significant promise for practical implementation,as it can satisfy the need for low-cost,high-sensitivity,and home-based quantitative detection for respiratory infectious diseases.

关 键 词：Au-Fe_(3)O_(4) dumbbell-like nanoparticles Photothermal effect LFIA S protein 

分 类 号：O657.3[理学—分析化学] TB383.1[理学—化学] R511[一般工业技术—材料科学与工程]