中空纤维离心超滤-高效液相色谱法测定ICU患者比阿培南的谷浓度  

Hollow fiber centrifugal ultrafiltration-high performance liquid chromatography for the determination of trough concentration of biapenem in intensive care unit patients

作　　者：陶兴隆 张甜甜[1] 张宇 武玺坤 马晓松 吴瑕[1] 董维冲 宋宁[2] 张志清[1] TAO Xinglong;ZHANG Tiantian;ZHANG Yu;WU Xikun;MA Xiaosong;WU Xia;DONG Weichong;SONG Ning;ZHANG Zhiqing(Department of Pharmacy,2.Department of Infectious Diseases,3.Department of Preventive Healthcare,Second Hospital of Hebei Medical University,Hebei Shijiazhuang 050200,China)

机构地区：[1]河北医科大学第二医院药学部,河北石家庄050200 [2]河北医科大学第二医院感染性疾病科,河北石家庄050200 [3]河北医科大学第二医院预防保健处,河北石家庄050200

出　　处：《中国医院药学杂志》2024年第11期1260-1263,1304,共5页Chinese Journal of Hospital Pharmacy

基　　金：北京医卫健康公益基金会医学科学研究基金项目(编号:YWJKJJHKYJJ-ZLTC2301)。

摘　　要：目的:建立一种快速、简便、低成本的比阿培南血药浓度测定方法,并对ICU患者比阿培南谷浓度进行测定。方法:建立人血浆中比阿培南浓度的测定方法,并对其验证。前瞻性地收集2023年3—8月使用比阿培南的ICU患者8例,并测定其谷浓度。结果:比阿培南标准曲线为:y=0.1796x+0.0193(r=0.9999),0.2~50μg·m L^(-1),线性关系良好,定量下限为0.2μg·m L^(-1)。低、中、高3个质量浓度(0.5、5、40μg·m L^(-1))的绝对回收率平均分别为97.40%、99.43%和93.58%,方法回收率分别为100.55%、94.81%和97.41%。3个浓度的日间精密度、日内精密度均小于5.0%,使用本方法测得8例ICU患者的比阿培南谷浓度在0.51~1.79μg·m L^(-1)。ICU患者血药浓度测定结果表明,比阿培南对大肠杆菌具有较好疗效,对奇异变形杆菌、肺炎克雷伯菌和鲍曼不动杆菌引起感染的治疗效果可能不理想。结论:该方法简便、准确、重复性高、成本低,可用于比阿培南生物样品日常测定。可针对ICU感染患者进行治疗药物监测,及时调整用药方案。OBJECTIVE To establish a rapid,simple and low-cost method for determining the plasma concentration of biap⁃enem and measuring the trough concentration in intensive care unit(ICU)patients.METHODS A method for measuring the concentration of biapenem in human plasma was established and validated.From March 2023 to August 2023,8 ICU patients on a therapy of biapenem were prospectively recruited and their trough concentrations measured.RESULTS The standard curve of biapenem was:y=0.1796x+0.0193(r=0.9999),0.2—50μg·m L^(-1).Linear relationship was decent with a lower limit of quan⁃tification at 0.2μg·m L^(-1).The absolute recoveries of low,medium and high concentrations(0.5,5,40μg·m L^(-1))averaged 97.40%,99.43%and 93.58%and the method recoveries reached 100.55%,94.81%and 97.41%.For the above three concen⁃trations,intraday relative standard deviation(RSD)value was<5.0%and the concentration range of biapenem was 0.51—1.79μg·m L^(-1).The results of blood concentration determination in ICU patients have demonstrated that biapenem has a decent efficacy for Enterobacteriace.And its effect may not be ideal for infections caused by Proteus mirabilis,Klebsiella pneumoniae or Acinetobacter baumannii.CONCLUSION This method is simple,accurate,reproducible and inexpensive.It may be used for routine determination of biapenem biological samples.Therapeutic drug monitoring should be performed for ICU patients so that dosing regimen may be adjusted in time.

关 键 词：比阿培南 中空纤维离心超滤 治疗药物监测 

分 类 号：R969[医药卫生—药理学]