小红参对高胆碱膳食小鼠肠道菌群及代谢物短链脂肪酸的影响  

Effects of Rubia yunnanensis(Franch.)on intestinal flora and metabolites of short-chain fatty acids in mice on a high choline diet

作　　者：李高一舟 程江豪 陈普 段小花 LI Gaoyizhou;CHENG Jianghao;CHEN Pu;DUAN Xiaohua(Yunnan Key Laboratory of Dai and Yi Medi-cines,Yunnan University of Chinese Medicine,Yunnan Kunming 650500,China)

机构地区：[1]云南中医药大学云南省傣医药与彝医药重点实验室,云南昆明650500

出　　处：《中国医院药学杂志》2024年第11期1292-1297,共6页Chinese Journal of Hospital Pharmacy

基　　金：云南省教育厅科学研究基金项目(编号:2023Y0447);云南省傣医药与彝医药重点实验室开放课题(编号:2021KY002);云南省兴滇英才支持计划“青年人才”项目(编号:XDYC-QNRC-2022-0284)。

摘　　要：目的:研究小红参对高胆碱饮食C57BL/6J小鼠肠道菌群组成及代谢物短链脂肪酸的影响。方法:将18只C57BL/6J小鼠采用随机数字表法分为空白对照(Control)组、高胆碱膳食(CHO)组和小红参干预(RY)组,连续给药6周后,采用16S rRNA测序技术测定小鼠肠内粪便的肠道菌群组成,使用气相色谱-质谱联用法测定肠内粪便中短链脂肪酸含量。结果:16S rRNA测序结果显示:CHO组肠道菌群多样性降低,与CHO组相比,RY组肠道菌群多样性增加,肠道微生态得到改善;RY组中Lachnospiraceae_NK4A136_group、Lactobacillus和Bifidobacterium的相对丰度显著高于CHO组,RY组中Desulfovibrio的相对丰度显著低于CHO组,RY组短链脂肪酸的含量也高于CHO组。结论:小红参可以优化肠道菌群的组成,改善肠道的微生态环境,增加短链脂肪酸含量,为小红参的临床应用提供理论支持。OBJECTIVE To explore the effects of Rubia yunnanensis(Franch.)on intestinal flora composition and metabo⁃lites of short-chain fatty acids in C57BL/6J mice on a high-choline diet.METHODS Eighteen C57BL/6J mice were randomized into three groups of blank control(Control),high choline diet(CHO)and Rubia yunnanensis(Franch.)intervention(RY).After 6-week continuous dosing,the composition of intestinal flora in intestinal feces of mice was determined by 16S rRNA sequencing and the content of short-chain fatty acids in intestinal feces measured by gas chromatography-mass spectrometry.RESULTS 16S rRNA sequencing results showed that intestinal flora diversity of CHO group declined while intestinal flora diver⁃sity of RY group spiked as compared with CHO group.And intestinal microecology improved.The relative abundance of Lachno⁃spiraceae_NK4A136_group,Lactobacillus and Bifidobacterium was significantly higher in RY group than that in CHO group.And the relative abundance of Desulfovibrio was significantly lower in RY group than that in CHO group.The content of shortchain fatty acids was also higher in RY group than that in CHO group.CONCLUSION Rubia yunnanensis(Franch.)may opti⁃mize the composition of intestinal flora,improve the microecological environment of intestinal tract,boost the content of shortchain fatty acids through enriching the abundance of beneficial bacteria and increasing the content of short-chain fatty acids.Thus it provides theoretical rationales for future clinical application of Rubia yunnanensis(Franch.).

关 键 词：小红参 高胆碱膳食 肠道菌群 短链脂肪酸 

分 类 号：R285.5[医药卫生—中药学]