基于钳夹损伤腹主动脉建立动脉内膜增生的小鼠模型  

Establishment of an intimal hyperplasia mouse model based on infrarenal abdominal aorta clamping

作　　者：姜榆 毛由军 Jiang Yu;Mao Youjun(Department of Vascular Surgery,Changzhou Second People’s Hospital,Changzhou 213164,China)

机构地区：[1]常州市第二人民医院血管外科,常州213164

出　　处：《中华实验外科杂志》2024年第5期925-928,共4页Chinese Journal of Experimental Surgery

摘　　要：目的:构建一种稳定、高效的小鼠动脉内膜增生模型。方法:设计改良的钳夹损伤方案,将其运用于小鼠(C57BL/6小鼠33只,雄性,8~12周,由汉堡大学附属艾本多夫医学中心动物房提供)腹主动脉,24只小鼠通过随机数表随机分为2组(术后28天):对照组和手术组,每组12只;剩下9只随机分成3组,对照组、术后即刻组和术后4天组,每组3只。对照组小鼠仅麻醉处理,手术组小鼠进行腹主动脉钳夹损伤,术后定期取小鼠腹主动脉,进行苏木精-伊红(HE)染色、弹力纤维(EVG)染色、血小板内皮细胞黏附分子(CD31)免疫荧光染色等组织学检测,分析观察结果,包括增生病变的分布,内、外弹性膜周长、中膜面积、免疫荧光表达等,组间比较采用t检验。结果:全部手术小鼠均存活,其中92%(11/12)的手术小鼠产生了内膜增生,且HE染色结果表示增生内膜多发生于近心端的腹主动脉。动脉内弹性膜周长手术组高于对照组[(1769.21±77.61)μm比(1616.16±55.05)μm,t=5.202,P<0.01],动脉外弹性膜周长手术组高于对照组[(1836.75±97.10)μm比(1645.03±80.19)μm,t=5.270,P<0.01],动脉中膜面积手术组高于对照组[(38079.89±8711.61)μm比(29808.88±2753.53)μm,t=3.140,P<0.01]。CD31染色结果显示损伤动脉出现了内皮剥脱和再内皮化的过程。结论:该研究建立了一种新的更为高效稳定的用于研究动脉内膜增生的小鼠模型。Objective To set up a stable,efficient infrarenal abdominal aorta intimal hyperplasia mouse model.Methods An existing clamping method was modified and applied in mouse(33 C57BL/6 mice,male,8-12 weeks old)infrarenal abdominal aorta.The 24 mice were randomly divided into 2 groups(28 days after surgery,n=12):control group and clamping group.The rest 9 mice were randomly divided into 3 groups(n=3):control group,0 day and 4 days after surgery.The control group only underwent anesthesia and the clamping group underwent infrarenal abdominal aorta clamping.The mouse infrarenal abdominal aortas were taken after animal sacrifice for hematoxylin and eosin(HE)staining,verhoeff’s van Gieson(EVG)staining and immunofluorescence staining of cluster of differentiation 31(CD31)for histology analysis.The distribution of lesions was observed,the circumference of external,internal elastic laminae and the media area were measured and compared.The signal of CD31 immunofluorescence staining was observed.The t-test was used for data analysis.Results All mice survived from surgery and 92%(11/12)of the clamped mice developed intimal hyperplasia lesion 28 days after surgery.The lesion distribution analysis showed that most of the lesions were located in the approximal part of the aorta.The circumference of the internal elastic lamina in the clamping group was longer than the control group[(1769.21±77.61)μm vs.(1616.16±55.05)μm,t=5.202,P<0.0001],the circumference of the external elastic lamina in the clamping group was longer than the control group[(1836.75±97.10)μm vs.(1645.03±80.19)μm,t=5.270,P<0.0001]and the media area in the clamping group was also larger than the control group[(38079.89±8711.61)μm vs.(29808.88±2753.53)μm,t=3.140,P<0.005].The CD31 staining results showed the process of endothelium denudation and re-endothelialization.Conclusion The study has established a new mouse which is more stable and efficient for aorta intimal hyperplasia research.

关 键 词：腹主动脉 内膜增生 损伤 动物模型 

分 类 号：R654.3[医药卫生—外科学] R-332[医药卫生—临床医学]