Direct Analysis of Whole Blood by a Disposable Monolithic Column Mass Spectrometry Analysis Kit  

作　　者：Wenxin Wang Jin Han Yutong Zheng Haijie Wang Chengbiao Zhu Yongyi Li Yuqi Feng Zhenwei Wei Xiangyu Wang 

机构地区：[1]College of Chemistry and Molecular Science,Wuhan University,Wuhan,Hubei 430072,China [2]School of Public Health,Wuhan University,Wuhan,Hubei 430072,China [3]Schoolof Bioengineering and Health,Wuhan Textile University,Wuhan,Hubei 430200,China

出　　处：《Chinese Journal of Chemistry》2024年第10期1073-1078,共6页中国化学（英文版）

基　　金：financially supported by the National Natural Science Foundation of China(No.22104112);the startup foundation of Wuhan University(No.600460076).

摘　　要：A monolithic column-based mass spectrometry(MS)analysis kit was prepared for whole blood analysis with MS.The kit is disposable and can be used for purification,storage,transportation and direct analysis of whole blood.The kit mainly consists of a capillary for quantitative microsampling,a cation exchange monolithic column for purification and storage,and a syringe for loading sample.This kit is very friendly to various users that one can easily siphon the blood in the kit followed by rapid clean-up.We established a quantitative method using the kit with a limit detection as low as 0.33 nmol/L,and achieved more than five orders of magnitude enhancement in sensitivity compared to direct nanoelectrospray ionization MS analysis.The column can avoid analyte exposure to environment,which helps the storage of the sample for laboratory analysis.The relative standard deviation of immediate blood analysis and storage blood analysis within 10 d was less than 10%.This method has been successfully applied to the quantitative analysis of procainamide hydrochloride in 2μL rat blood.These results indicate that this disposable kit does have the potential to achieve highly sensitive quantitative MS analysis in biological samples,which is expected to become a cost-effective and powerful tool for in vitro diagnostics.

关 键 词：Clinical Ms Whole blood analysis Porous polymers Matrix isolation Trace analysis 

分 类 号：O65[理学—分析化学]