检索规则说明:AND代表“并且”;OR代表“或者”;NOT代表“不包含”;(注意必须大写,运算符两边需空一格)
检 索 范 例 :范例一: (K=图书馆学 OR K=情报学) AND A=范并思 范例二:J=计算机应用与软件 AND (U=C++ OR U=Basic) NOT M=Visual
作 者:覃艮红 袁洪波 王卓妮 史冰柯 范洋洋 王丽[1] 张猛[2] 涂洪涛[1,3] 徐超 侯珲 QIN Genhong;YUAN Hongbo;WANG Zhuoni;SHI Bingke;FAN Yangyang;WANG Li;ZHANG Meng;TU Hongtao;XU Chao;HOU Hui(Zhengzhou Fruit Research Institute,Chinese Academy of Agricultural Sciences,Zhengzhou 450009,China;College of Plant Protection,Henan Agricultural University,Zhengzhou 450009,China;Zhongyuan Research Center,Chinese Academy of Agricultural Sciences,Xinxiang,Henan 453004,China)
机构地区:[1]中国农业科学院郑州果树研究所,郑州450009 [2]河南农业大学植物保护学院,郑州450009 [3]中国农业科学院中原研究中心,河南新乡453004
出 处:《园艺学报》2024年第6期1403-1412,共10页Acta Horticulturae Sinica
基 金:中央级公益性科研院所基本科研业务费专项(1610192023311,ZGS202205);中国农业科学院科技创新工程项目(CAAS-ASTIP-2016-RIP);河南省重点研发与推广专项(232102110052)。
摘 要:为了探索苹果轮纹病生物防治方法,分析了蜡样芽孢杆菌菌株G3-17挥发性有机化合物对苹果轮纹病菌的拮抗活性。平皿对扣试验显示,在前期研究基础上菌株G3-17不同浓度的挥发物均能显著抑制苹果轮纹病菌的生长。顶空固相微萃取—气相色谱—质谱联用分析显示,菌株G3-17主要分泌6种挥发性有机化合物,其中苯甲醛对苹果轮纹病菌的抑制效果最好,在浓度为125~625μL·L^(-1)范围内抑制率为57.24%~100.00%。苯甲醛处理还能破坏苹果轮纹病菌细胞膜的完整性,并能诱导苹果果实病程相关蛋白基因(Md PR1和Md PR5)的表达。防效试验结果显示,苯甲醛处理能有效防治苹果轮纹病,在浓度为125、250和500μL·L^(-1)时的防效分别为39.80%、84.77%和90.67%。研究表明,蜡样芽孢杆菌菌株G3-17挥发物及主要成分苯甲醛对苹果轮纹病具有较好的防治潜力。The purpose of this study is to analyze the antagonistic activity of volatile organic compounds(VOCs)of Bacillus cereus strain G3-17 against Botryosphaeria dothidea,and to explore the biological control methods of apple ring rot.The plate-to-plate experiment showed that the volatiles of Bacillus cereus strain G3-17 at different concentrations could significantly inhibit the growth of Botryosphaeria dothidea.Headspace solid-phase microextraction-gas chromatography-mass spectrometry analysis showed that the strain G3-17 mainly secreted six VOCs.Among them,benzaldehyde had the best inhibitory effect on Botryosphaeria dothidea,with an inhibition rate of 57.24%–100.00%in the concentration range of 125–625μL•L^(-1).Further results also showed that benzaldehyde treatment also destroyed the integrity of the cell membrane of Botryosphaeria dothidea.In addition,benzaldehyde treatment could induce the expression of apple fruit pathogenesis-related protein genes(MdPR1 and MdPR5).Benzaldehyde treatment also could effectively control apple ring rot caused by Botryosphaeria dothidea,and the control effects were 39.80%,84.77%and 90.67%at the concentrations of 125,250 and 500μL•L^(-1),respectively.In summary,the volatiles of Bacillus cereus strain G3-17 and its main VOCs have good control potential against apple ring rot.
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在链接到云南高校图书馆文献保障联盟下载...
云南高校图书馆联盟文献共享服务平台 版权所有©
您的IP:216.73.216.248