Genome engineering of the human gut microbiome  

作　　者：Linggang Zheng Juntao Shen Ruiyue Chen Yucan Hu Wei Zhao Elaine Lai-Han Leung Lei Dai 

机构地区：[1]Dr Neher's Biophysics Laboratory for Innovative Drug Discovery/State Key Laboratory of Quality Research in Chinese Medicine,Macao University of Science and Technology,Taipa,Macao 999078,China [2]CAS Key Laboratory of Quantitative Engineering Biology,Shenzhen Institute of Synthetic Biology,Shenzhen Institutes of Advanced Technology,Chinese Academy of Sciences,Shenzhen 518055,China [3]University of Chinese Academy of Sciences,Beijing 100049,China [4]Cancer Center,Faculty of Health Science,University of Macao,Macao 999078,China [5]MOE Frontiers Science Center for Precision Oncology,University of Macao,Macao 999078,China

出　　处：《Journal of Genetics and Genomics》2024年第5期479-491,共13页遗传学报（英文版）

基　　金：National Key R&D Program of China(2019YFA0906700);Guangdong Basic and Applied Basic Research Foundation(2020A1515110184);Dr.Neher's Biophysics Laboratory for Innovative Drug Discovery(001/2020/ALC),regular grants(0056/2020/AMJ&0063/2022/A2)from Macao Science and Technology Development Fund;2020 Young Qihuang Scholar funded by National Administration of Traditional Chinese Medicine and also financially supported by the Start-up Research Grant of University of Macao(SRG2022-00020-FHS)and the Faculty of Health Sciences,University of Macao.

摘　　要：The human gut microbiome,a complex ecosystem,significantly influences host health,impacting crucial aspects such as metabolism and immunity.To enhance our comprehension and control of the molecular mechanisms orchestrating the intricate interplay between gut commensal bacteria and human health,the exploration of genome engineering for gut microbes is a promising frontier.Nevertheless,the complexities and diversities inherent in the gut microbiome pose substantial challenges to the development of effective genome engineering tools for human gut microbes.In this comprehensive review,we provide an overview of the current progress and challenges in genome engineering of human gut commensal bacteria,whether executed in vitro or in situ.A specific focus is directed towards the advancements and prospects in cargo DNA delivery and high-throughput techniques.Additionally,we elucidate the immense potential of genome engineering methods to enhance our understanding of the human gut microbiome and engineer the microorganisms to enhance human health.

关 键 词：Non-model gut commensals Genomeediting reagents Cargo delivery High-throughput genome engineering 

分 类 号：R542.22[医药卫生—心血管疾病]