精浆中重金属含量和精子质量与复发性流产的关系  

Correlation between heavy metal content and sperm quality in male seminal plasma and recurrent spontaneous abortion

作　　者：包华琼 田美平 丁杰 廖会姝 熊英 杨柳 高鹏飞 Bao Huaqiong;Tian Meiping;Ding Jie;Liao Huishu;Xiong Ying;Yang Liu;Gao Pengfei(Affiliated Hospital of Chongqing Institute for Population and Family Planning,Chongqing Key Laboratory of Birth Defects and Reproductive Health,Chongqing 400020,China;Key Lab of Urban Environment and Health,Institute of Urban Environment,Chinese Academy of Sciences,Xiamen 361021,Fujian,China;Shanghai WeHealth Biomedical Technology Co.,Ltd.,State Key Laboratory of Genetic Engineering,School of Life Sciences,Fudan University,Shanghai 200433,China)

机构地区：[1]重庆市人口和计划生育科学技术研究院附属医院,重庆市出生缺陷与生殖健康重点实验室,重庆400020 [2]中国科学院城市环境研究所,福建厦门361021 [3]上海韦翰斯生物医药科技有限公司,复旦大学生命科学院基因工程重点实验室,上海200433

出　　处：《中国男科学杂志》2024年第2期50-55,共6页Chinese Journal of Andrology

基　　金：重庆市自然科学基金面上项目(cstc2021jcyj⁃msxmX0468);上海韦翰斯生物医药科技有限公司横向项目(2021yjy⁃hxkt⁃0002)。

摘　　要：目的探讨男性精浆中重金属含量和精子质量与复发性流产的相关性。方法选取2021年10月至2022年12月在重庆市人口和计划生育研究院附属医院就诊的复发性流产夫妇中男性配偶122例(流产组),两年内正常生育男性97例(生育组)为受试者。所有受试者填写知情同意书和健康调查表;提供精液标本进行精液常规、精子活力、精子形态、精子DNA碎片指数(DFI)和精浆重金属元素含量检测。结果(1)采用电感耦合等离子体质谱法(ICP⁃MS)对219份精浆分别检测锌(Zn)、铅(Pb)、砷(As)、铬(Cr)、钴(Co)、镍(Ni)、镓(Ga)、硒(Se)、锶(Sr)、锡(Sn)、锑(Sb)、钡(Ba)、铊(TI)、钛(Ti)、钒(V)、锰(Mn)、铝(Al)、铁(Fe)等18种重金属含量,结果显示流产组精浆中Cr、Se、Sb、TI、Ti等5种金属元素含量均显著高于生育组,其差异具有统计学意义(P<0.05);流产组和生育组中Zn、Pb、As等13种重金属含量未见统计学差异。(2)流产组和生育组精液量、精子浓度、前向运动精子百分率、精子形态正常率差异不显著;但流产组精子DNA碎片率(DFI)显著高于对照组(P<0.05)。(3)矫正流产组年龄、BMI、吸烟、饮酒、压力、体育运动等混杂因素后,显示精浆样本中Ti和Fe为精子DNA碎片率(DFI)的影响因素,其OR值及95%CI分别为4.416(1.39~14.028)、0.744(0.589~0.938)。结论复发性流产男性精子DFI显著高于正常生育男性,精浆中的Ti可能是男性精子DFI异常的风险因素,而Fe对精子DFI具有潜在保护作用。Objective To investigate the correlation between heavy metal content in male seminal plasma and sperm quality and recurrent spontaneous abortion(RSA).Methods Total of 122 male spouses in couples with recurrent spontaneous abortion from October 2021 to December 2022 were enrolled into the abortion group,and 97 male participants with normal fertility within two years into the fertility group.All subjects completed informed consent and health questionnaire;Their semen samples were collected for semen quality analysis including sperm motility,sperm morphology,sperm DNA fragmentation index(DFI),and seminal plasma heavy metal concentration.Results 1.The concentrations of 18 heavy metals,including zinc(Zn),lead(Pb),arsenic(As),chromium(Cr),copper(Cu),nickel(Ni),gallium(Ga),selenium(Se),strontium(Sr),tin(Sn),antimony(Sb),barium(Ba),titanium(Ti),vanadium(V),manganese(Mn),aluminum(Al),and iron(Fe),were analyzed in 219 semen samples using inductively coupled plasma mass spectrometry(ICP⁃MS).The results showed that the levels of Cr,Se,Sb,Ti,and Ti in the semen of the abortion group were significantly higher than those in the fertility group,and the differences were statistically significant(P<0.05).However,there were no significant differences in the levels of 13 heavy metals,including zinc,lead,and arsenic,between the two groups.2.No significant differences were found in semen volume,sperm concentration,forward sperm and normal morphology between the abortion and fertility groups;however,sperm DNA fragmentation rate(DFI)in the abortion group was significantly higher than that in the fertility groups(P<0.05).3.After normalizing the confounding factors such as age,BMI,smoking,alcohol consumption,stress,and physical exercise,Ti and Fe in seminal plasma samples were found to be associated with sperm DNA fragmentation rate(DFI).The OR value(95%CI)was 4.416(1.39~14.028)for Ti,and 0.744(0.589~0.938)for Fe.Conclusion DFI of the abortion group is significantly higher than that of fertility groups.Ti level in seminal plasma may be

关 键 词：重金属 精子质量 复发性流产 精子DNA碎片指数(DFI) 

分 类 号：R698.2[医药卫生—泌尿科学] R321.1[医药卫生—外科学]