慢性光化性皮炎患者外周血T细胞亚群及皮损白细胞介素-17、叉头状螺旋转录因子3的表达  

A study on the clinical significance of the peripheral blood T cell subsets percentage and the expression of IL-17 and FOXP3 in skin lesions in patients with chronic actinic dermatitis

作　　者：李涛 黄静 孙素姣 陈文秋 李惠娟 邓娜 苗宏建 杨达勇 LI Tao

机构地区：[1]大理大学第一附属医院,云南大理671000

出　　处：《中医临床研究》2024年第16期1-8,共8页Clinical Journal Of Chinese Medicine

基　　金：云南省地方本科高校基础研究联合专项(202001BA070001-073)。

摘　　要：目的:检测慢性光化性皮炎(Chronic Actinic Dermatitis,CAD)患者外周血辅助性T细胞(T Helper Cells,Th)17、调节性T细胞(Regulatory T Cells,Treg)、Th1、Th2细胞百分比以及皮损中白细胞介素(Interleukin,IL)-17、叉头状螺旋转录因子3(Forkhead Helix Transcription Protein 3,FOXP3)的表达,探究其变化趋势及相关临床意义。方法:外周血T细胞亚群的检测:以大理大学第一附属医院皮肤科符合CAD诊断标准的患者20例为试验1组,以健康体检科符合研究标准的20例健康体检者作为对照1组。通过流式细胞分析仪检测外周血中Th1、Th2、Th17、Treg细胞的百分比,计算Th1/Th2和Th17/Treg值。采用相应统计学方法进行统计分析。皮损IL-17、FOXP3的检测:选取大理大学第一附属医院皮肤科符合CAD诊断标准的患者20例作为试验2组,以手术门诊切痣患者切下的痣组织周围正常皮肤组织20例作为对照2组。通过石蜡切片免疫荧光实验检测CAD皮损和正常皮肤组织中IL-17、FOXP3的表达。采用相应的统计学方法进行统计分析。结果:①试验1组外周血Th1、Th2、Th17、Treg百分比分别为(25.81±6.52)%、(42.79±9.66)%、(12.34±5.15)%、(8.32±3.06)%;对照1组外周血Th1、Th2、Th17、Treg百分比分别为(12.59±2.69)%、(50.88±9.06)%、(12.74±5.09)%、(10.42±2.07)%;②试验1组Th1/Th2和Th17/Treg值分别为0.58(0.48,0.74)和1.65(1.00,2.21);对照组Th1/Th2和Th17/Treg值分别为0.26(0.21,0.31)和1.23(0.82,1.45);③试验2组皮损中IL-17和FOXP3的阳性率分别为(85.91±11.73)%和3.60(1.97,4.83)%;对照1组皮损中IL-17和FOXP3的阳性率分别为(89.85±8.18)%和1.32(0.85,2.79)%。结论:CAD患者非急性发作期外周血中Treg低表达、Th1高表达,皮损中FOXP3高表达,其发病可能与外周血Th17/Treg、Th1/Th2表达失衡及皮损IL-17/FOXP3的表达失衡有关,而Th17/Treg、Th17百分比及皮损中IL-17表达的差异无统计学意义。Objective:To detect the percentage of Th17,Treg,Th1 and Th2 cells in peripheral blood of patients with chronic actinic dermatitis(CAD)and the expression of IL-17 and FOXP3 in skin lesions,and to explore the change trend and related clinical significance.Methods:Peripheral blood T cell subsets were detected.A total of 20 patients who met CAD diagnostic criteria in dermatology department of the First Affiliated Hospital of Dali University were selected as the experimental 1 group.The control 1 group was selected from 20 healthy subjects who met the study criteria in the physical examination department.The percentage of Th1,Th2,Th17 and Treg cells in peripheral blood were detected by flow cytometry,and the ratio of Th1/Th2 and Th17/Treg were calculated.Corresponding statistical methods were used for statistical analysis.IL-17 and FOXP3 in skin lesions were detected.A total of 20 patients who met CAD diagnostic criteria in dermatology department of the First Affiliated Hospital of Dali University were selected as the experimental 2 group.Twenty cases of normal skin tissue around the nevus tissue were selected as the control 2 group.The expressions of IL-17 and FOXP3 in CAD lesions and normal skin tissues were detected by immunofluorescence assay in paraffin sections.Corresponding statistical methods were used for statistical analysis.Results:The percentages of Th1,Th2,Th17,Treg,Th1 and Th2 in peripheral blood in the experimental 1 group were(25.81±6.52)(42.79±9.66)(12.34±5.15)(8.32±3.06),respectively.The percentages of Th1,Th2,Th17 and Treg in peripheral blood in the health group were(12.59±2.69)(50.88±9.06)(12.74±5.09)(10.42±2.07),respectively.The ratios of Th1/Th2 and Th17/Treg in the experimental 2 group were 0.58[0.48,0.74]and 1.65[1.00,2.21],respectively.In the control 1 group,the ratios of Th1/Th2 and Th17/Treg were 0.26[0.21,0.31]and 1.23[0.82,1.45],respectively.The positive rates of IL-17 and FOXP3 in skin lesions in the experimental 2 group were(85.91±11.73)and 3.60[1.97,4.83],respectively.The posit

关 键 词：慢性光化性皮炎 白细胞介素-17 叉头状螺旋转录因子3 T细胞亚群 

分 类 号：R75[医药卫生—皮肤病学与性病学]