颈椎康复丸治疗蛋白聚糖诱导小鼠骨关节炎研究  

Therapeutic effect of Jingzhui Kangfu pills against osteoarthritis induced by proteoglycan in mice

作　　者：刘洋[1] 范峥[1] 王宏蕾[1] 杨颖 国琦 常馨予[1] 韩丽娟[1] 李文喆[1] 吴剑坤[1] LIU Yang;FAN Zheng;WANG Honglei;YANG Ying;GUO Qi;CHANG Xinyu;HAN Lijuan;LI Wenzhe;WU Jiankun(Department of Pharmacy,Beijing Hospital of Traditional Chinese Medicine,Capital Medical University,Beijing 100010,China)

机构地区：[1]首都医科大学附属北京中医医院药学部,北京100010

出　　处：《中国药物警戒》2024年第6期651-654,659,共5页Chinese Journal of Pharmacovigilance

基　　金：国家重点研发计划(2019YFC1710604);毛克臣全国名老中医传承工作室;临床中药学学科建设项目。

摘　　要：目的研究中药复方颈椎康复丸治疗蛋白聚糖诱导小鼠骨关节炎的影响,为扩大该药的适应证及临床应用提供参考。方法采用Balb/c小鼠进行实验,将小鼠随机分为正常组,模型组,颈椎康复丸高、低剂量(6、3 g·kg^(-1)·d^(-1))组。全部小鼠(正常组除外)腹腔注射0.2 mL DDA佐剂(含100μg蛋白聚糖)造模;正常组给予0.2 mL生理盐水。首次免疫后,每隔2周进行关节炎评估和体重监测直到第14周。应用HE染色和番红固绿染色观察关节组织病理形态。应用ELISA法测定小鼠血清炎症因子IL-1β、IL-6、TNF-α和IL-17A。结果颈椎康复丸对小鼠骨关节炎有明显的抑制作用,能降低关节炎性评分和肿胀度。镜下观察,颈椎康复丸能保护小鼠关节组织,减少炎性浸润。颈椎康复丸能降低小鼠血清中炎症因子IL-1β、IL-6、TNF-α和IL-17A水平。结论颈椎康复丸对蛋白聚糖诱导的小鼠骨关节炎有改善作用。Objective To study the therapeutic effects of Jingzhui Kangfu pills against proteoglycan-induced osteoarthritis in mice and to provide data for increasing the indications and clinical applications of this drug.Methods Balb/c mice were randomly divided into the normal group,model group,high dose(6,3 g·kg^(-1)·d^(-1))group and low dose group.All the mice(except the normal group)were intraperitoneally injected with 0.2 mL DDA adjuvant(containing 100μg proteoglycan)to establish a model.The normal group was given 0.2 mL normal saline.After initial immunization,arthritis was assessed and weight was monitored every 2 weeks until the 14th week.The histopathologic morphology of joints was observed via HE staining and Safranine O-Fast Green staining.Levels of serum inflammatory factors IL-1β,IL-6,TNF-αand IL-17A were determined by ELISA.Results Jingzhui Kangfu pills could significantly mitigate osteoarthritis in mice,and lower the score of arthritics and degree of swelling.Under microscopic observation,Jingzhui Kangfu pills could protect the joint tissue of mice and reduce inflammatory infiltration and significantly reduce the levels of serum inflammatory factors IL-1β,IL-6,TNF-αand IL-17A.Conclusion Jingzhui Kangfu pills can improve proteoglycan-induced osteoarthritis in mice.

关 键 词：颈椎康复丸 骨关节炎 炎症因子 蛋白聚糖 中药复方 小鼠 疗效 

分 类 号：R961[医药卫生—药理学] R971[医药卫生—药学]