佛芍颗粒对小鼠肠道菌群及乳糖酶活性的调控作用研究  

Roles of Foshao granules in regulating gut microbiota and lactase activity in mice

作　　者：李舒冉 郭姗姗[1] 赵荣华[1] 包蕾[1] 孙静[1] 耿子涵 鲍岩岩[1] 庞博[1] 陈梦苹 吕耀中 王振中[2,3] 崔晓兰 王红梅[2,3] LI Shuran;GUO Shanshan;ZHAO Ronghua;BAO Lei;SUN Jing;GENG Zihan;BAO Yanyan;PANG Bo;CHEN Mengping;LYU Yaozhong;WANG Zhenzhong;CUI Xiaolan;WANG Hongmei(Biosecurity Laboratory,Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China;National Key Laboratory of Process Control and Intelligent Manufacturing Technology of Traditional Chinese Medicine Pharmaceuticals,Lianyungang Jiangsu 222000,China;Jiangsu Kangyuan Pharmaceutical Co.,Ltd,Lianyungang Jiangsu 222000,China)

机构地区：[1]中国中医科学院中药研究所生物安全实验室,北京100700 [2]中药制药过程控制与智能制造技术全国重点实验室,江苏连云港222000 [3]江苏康缘药业股份有限公司,江苏连云港222000

出　　处：《中国药物警戒》2024年第6期655-659,共5页Chinese Journal of Pharmacovigilance

基　　金：国家自然科学基金资助项目(82141206);中央级公益性科研院所基本科研业务费专项资金资助(ZZ15-YQ-035;ZXKT21031);中国中医科学院科技创新项目(CI2021B015)。

摘　　要：目的明确佛芍颗粒对小鼠肠道菌群失调及乳糖酶活性的调节作用,为临床应用提供基础研究数据支持。方法实验小鼠按体质量随机分为正常组,模型组,吗丁啉组(12 mg·kg^(-1)),四逆散组(3 g·kg^(-1)),佛芍颗粒高剂量组(48 g生药·kg^(-1))、佛芍颗粒中剂量组(24 g生药·kg^(-1))、佛芍颗粒低剂量组(12 g生药·kg^(-1)),共7组,每组10只,雌雄各半。采用头孢拉定胶囊和硫酸庆大霉素(2∶1)混合物灌胃诱导肠道菌群失调模型小鼠,通过检测小鼠肠道总厌氧菌、乳酸杆菌、双歧杆菌、大肠杆菌数量及结肠黏膜乳糖酶活性水平,综合评价佛芍颗粒对菌群失调和乳糖酶活性的调控作用。结果佛芍颗粒可以明显增加总厌氧菌(P<0.01)、乳酸杆菌(P<0.01)、双歧杆菌(P<0.01)及大肠杆菌(P<0.01)的数量,并提高结肠黏膜乳糖酶活性。结论佛芍颗粒可以改善头孢拉定胶囊和硫酸庆大霉素诱导的菌群失调和乳糖酶活性降低,为佛芍颗粒的临床应用提供参考。Objective To study the effect of Foshao granules on the gut microbiota and lactase activity of mice.Methods Experimental rats were randomly divided into seven groups according to their body weight:the normal control group,model control group,domperidone control group(12 mg·kg^(-1)),Sini powder control group(3 g·kg^(-1)),Foshao granules high dosage group(48 g crude drug·kg^(-1)),medium dosage group(24 g crude drug·kg^(-1)),and low dosage group(12 g crude drug·kg^(-1)),with 10 mice in each.A model of flora imbalance mice was induced by a mixture of cefradine capsules and gentamycin sulfate(2:1).By measuring the number of total anaerobic bacteria,Lactobacillus,bifidobacteria,Escherichia coli,the extent to which Foshao granules regulated the gut microbiota and lactase activity of mice was evaluated.Results Foshao granules could significantly increase the number of total anaerobic bacteria(P<0.01),Lactobacillus(P<0.01),bifidobacteria(P<0.01)and Escherichia coli(P<0.01),and elevated the colonic mucosal lactase activity.Conclusion Foshao granules can improve flora imbalance and the reduction of lactase activity caused by cefradine and gentamicin sulphate.This study is expected to provide data for clinical applications of Foshao granules.

关 键 词：佛芍颗粒 头孢拉定 庆大霉素 菌群失调 乳糖酶活性 肠道 小鼠 

分 类 号：R994.11[医药卫生—毒理学]