抗类风湿性关节炎方剂通痹化瘀膏的药效物质基础研究  

Study on the anti-rheumatic arthritis constituents of Tongbihuayu Ointment

作　　者：杨敏飞 刘娜娜[2] 黄小强 职利琴 鲍和 刘杏[4] 王瑞 毛宇恒[2] 荣真吉 于春萍 王儒月 高阳 张海龙[2] YANG Minfei;LIU Na’na;HUANG Xiaoqiang;ZHI Liqin;BAO He;LIU Xing;WANG Rui;MAO Yuheng;RONG Zhenji;YU Chunping;WANG Ruyue;GAO Yang;ZHANG Hailong(The Fifth Hospital of Xi’an,Xi’an 710082,China;School of Pharmacy,Xi’an Jiaotong University,Xi’an 710061,China;Department of Pharmacy,the Second Affiliated Hospital,Xi’an Jiaotong University,Xi’an 710004,China;Gaoling Maternal and Child Health Hospital of Xi’an,Xi’an 710200,China)

机构地区：[1]西安市第五医院,西安710082 [2]西安交通大学药学院,西安710061 [3]西安交通大学第二附属医院药学部,西安710004 [4]高陵区妇幼保健院,西安710200

出　　处：《西北药学杂志》2024年第4期5-10,共6页Northwest Pharmaceutical Journal

基　　金：国家自然科学基金项目(编号:81673564,81102382);陕西省自然科学基金重点项目(编号:2019JZ-01);陕西省中医药管理局项目(编号:SZY-KJCYC-2023-080,2023-ZDYJSY009,2021-ZZ-JC002);西安市科技局项目(编号:24YXYJ0018,24YXYJ0021,22YXYJ0048,21YXYJ0117);西安市卫生健康委项目(编号:SZY202201,SJZZ202403)。

摘　　要：目的初步阐明抗类风湿性关节炎方剂通痹化瘀膏的药效物质基础,为其临床应用提供科学依据。方法用硅胶柱色谱、反相硅胶柱色谱、高效液相色谱等现代分离技术对通痹化瘀膏的有效部位进行分离纯化,结合化合物的理化性质和各种波谱技术对分离得到的化合物进行结构鉴定。结果从通痹化瘀膏有效部位中分离鉴定出14个化合物,分别是花椒素(1)、2-methoxyfuranoguaia-9-ene-8-one(2)、棕榈酸(3)、2-methoxy-5-acetoxy fruranogermacr-1(10)-en-6-one(4)、sarcophytol M(5)、ntetradecanyl oleate(6)、alismol(7)、2-methoxy-8,12-epoxygermacra-1(10),7,11-trien-6-one(8)、bis(2-ethyloctyl)phthalate(9)、(24S)-24-ethylcholest-5-en-3β-ol(10)、myrrhterpenoid E(11)、okilactomycin D(12)、bis(2-ethylhexyl)benzene-1,2-dicarboxylate(13)和5-羟甲基糠醛(14)。结论这14个化合物包括7个萜类、3个芳香族化合物、2个脂肪酸或酯、1个甾体和1个糠醛。本研究是首次对院内制剂通痹化瘀膏的药效物质基础进行研究。Objective To preliminarily study the effective substance of the active part of Tongbihuayu Ointment and to provide scien‐tific evidence for its clinic application.Methods The active part of Tongbihuayu Ointment was purified by silica gel column chroma‐tography,ODS column chromatography,HPLC and other methods.And the structures were characterized by spectroscopic analysis on the basis of their physicochemical properties.Results Fourteen compounds were isolated and identified as 1(-2-hydroxy-4,6-dime‐thoxyphenyl)ethan-1-one(1),2-methoxyfuranoguaia-9-ene-8-one(2),hexadecanoic acid(3),2-methoxy-5-acetoxy fruranoger‐macr-1(10)-en-6-one(4),sarcophytol M(5),n-tetradecanyl oleate(6),alismol(7),2-methoxy-8,12-epoxygermacra-1(10),7,11-trien-6-one(8),bis(2-ethyloctyl)phthalate(9),(24S)-24-ethylcholest-5-en-3β-ol(10),myrrhterpenoid E(11),okilactomy‐cin D(12),bis(2-ethylhexyl)benzene-1,2-dicarboxylate(13)and 5(-hydroxymethyl)furan-2-carbaldehyde(14).Conclusion These compounds included 7 terpenoids,3 phenyl compounds,2 fatty acids or esters,1 steroid and 1 furaldehyde.This is the first time to study the effective substance of Tongbihuayu Ointment.

关 键 词：院内制剂 通痹化瘀膏 类风湿性关节炎 活性部位 化学成分 

分 类 号：R284.1[医药卫生—中药学]