气相色谱法测定五加皮中7种掺伪香精  

Determination of 7 adulterated essence in Acanthopanacis Cortex by gas chromatography

作　　者：王锋[1] 崔艳华 王冰洁 顾璇 邹子爵 李灿 WANG Feng;CUI Yanhua;WANG Bingjie;GU Xuan;ZOU Zijue;LI Can(3201 Hospital of Xi’an Jiaotong University Health Science Center,Hanzhong 723000,China;Hunan Province Institute of Product and Goods Quality Inspection,Changsha 410007,China)

机构地区：[1]西安交通大学医学部附属三二〇一医院,汉中723000 [2]湖南省产商品质量检验研究院,长沙410007

出　　处：《西北药学杂志》2024年第4期11-15,共5页Northwest Pharmaceutical Journal

基　　金：湖南省自然科学基金项目(编号:2022JJ90024);食品安全快速检测与溯源关键技术研究与示范项目(编号:2020SK2128)。

摘　　要：目的建立气相色谱法测定五加皮中7种香精成分的分析方法。方法对提取溶剂、分流比及色谱柱型号等参数进行考察,利用氢火焰离子化检测器(flame ionization detector,FID)检测,采用外标法定量分析。结果将乙酸乙酯作为提取溶剂,CD624毛细管色谱柱分离,分流比为5∶1条件最优,7种香精成分能实现完全分离,线性关系良好,相关系数r均大于0.999。平均加标回收率为87.33%~93.66%,相对标准偏差(n=6)均低于5%。结论该方法简单、快速、准确,可满足五加皮中7种香精成分的检测。Objective To establish a gas chromatographic method for the determination of 7 essence components in Acanthopanacis Cortex.Methods The parameters,such as extraction solvent,split ratio and chromatographic column model were examined,and a gas chromatographic method was established by using hydrogen flame ionization detector(FID)and external standard method for quantification.Results When ethyl acetate was used as the extraction solvent and CD-624 capillary column was used for separation,the split ratio of 5∶1 was the best,and the 7 essence components could be completely separated,with good linear relationship,r>0.999.The average recovery rate of the added standard was 87.33%—93.66%,and the relative standard deviation(n=6)was less than 5%.Conclusion The method is simple,rapid and accurate,and can be used to detect the 7 essence ingredients in Acanthopanax Cortex.

关 键 词：气相色谱法 五加皮 掺伪 香精 

分 类 号：R284.1[医药卫生—中药学]