银马解毒颗粒调控急性肺损伤小鼠肺部炎症的效果  

Effect of Yinma Jiedu Granules on regulating lung inflammation in acute lung injury mice

作　　者：宫帅 赵爽[2] 张翔鹏 刘守智[2] GONG Shuai;ZHAO Shuang;ZHANG Xiangpeng;LIU Shouzhi(Department of Respiratory and Critical Care Medicine,Chengdu 363 Hospital Affiliated to Southwest Medical University,Chengdu 610031,China;Department of Respiratory and Critical Care Medicine,West China Hospital,Sichuan University,Chengdu 610041,China;Clinical Medicine School of West China Hospital,Sichuan University,Chengdu 610041,China)

机构地区：[1]西南医科大学附属成都三六三医院呼吸与危重症医学科,成都610031 [2]四川大学华西医院呼吸与危重症医学科,成都610041 [3]四川大学华西医院临床医学院,成都610041

出　　处：《西北药学杂志》2024年第4期39-45,共7页Northwest Pharmaceutical Journal

基　　金：国家高科技研究发展计划(863计划):白求恩公益基金项目(编号:HX-H2102037)。

摘　　要：目的分析银马解毒颗粒调控白化家鼠亚系(laboratory-bred strain,BALB/c)急性肺损伤小鼠肺部炎症的效果及安全性。方法选取6~8周龄C57BL/6J小鼠,用脂多糖(lipopolysaccharide,LPS)成功构建70只BALB/c急性肺损伤小鼠肺部炎症模型,并选取10只6~8周龄C57BL/6J小鼠作为空白组A。将70只模型小鼠随机分为7组(模型组B、银马解毒颗粒低剂量预防给药组C1、银马解毒颗粒高剂量预防给药组C2、银马解毒颗粒低剂量治疗组D1、银马解毒颗粒高剂量治疗组D2、银马解毒颗粒低剂量持续给药组E1和银马解毒颗粒高剂量持续给药组E2),每组10只。比较各组小鼠的药物毒性情况、肺泡灌洗液细胞计数,炎症因子和肺组织伊红染色法(hematoxylin-eosin staining,HE)染色情况。结果造模过程中共6只小鼠死亡,造模成功率为92.11%。与对照组比较,模型组的肺泡灌洗液炎症细胞数、炎症因子和HE染色炎症细胞浸润率均升高(P<0.05)。与正常肺组织比较,急性肺损伤模型鼠的肺组织HE染色可见肺泡间隔充血、水肿以及大量炎性细胞的浸润。成功造模的小鼠肺泡灌洗液细胞计数和炎症因子水平均高于A组,差异有统计学意义(P<0.05)。模型组小鼠的状态较差,表现为毛发耸立、抱团,进食量与饮水量明显减少,体质量减轻,大便干燥。各组急性肺损伤模型小鼠给予银马解毒颗粒灌胃1 d后,C2组、D2组和E2组小鼠的状态均较佳。造模8 d,模型组(B组)小鼠的死亡率达30%,显著高于其他各组(P<0.05);病理学上,与正常肺组织比较,急性肺损伤模型鼠的肺组织HE染色可见肺泡间隔充血、水肿以及大量炎性细胞的浸润。给予银马解毒颗粒后,肺泡间隔充血、水肿以及炎性细胞的浸润明显减少,且上述效果与银马解毒颗粒的剂量呈正比。在肺泡灌洗液细胞数方面,与A组比较,B组小鼠白细胞、中性粒细胞和淋巴细胞计数均明显增加。与E组和B组小鼠Objective To analyze the effect and safety of Yinma Jiedu Granules on regulating lung inflammation in laboratory-bred strain(BALB/c)acute lung injury mice.Methods 70 C57BL/6J male mice of 6-8 weeks old were selected to construct BALB/c acute lung injury inflammation model by using lipopolysaccharide(LPS),and the other 10 C57BL/6J male mice of 6-8 weeks old were used as blank group A for the control.The 70 model mice were randomly divided into 7 groups(model group B,low-dose prophylactic treatment group C1 of Yinma Jiedu Granules,high-dose prophylactic treatment group C2 of Yinma Jiedu Granules,lowdose treatment group D1 of Yinma Jiedu Granules,high-dose treatment group D2 of Yinma Jiedu Granules,low-dose continuous treatment group E1 of Yinma Jiedu Granules,and high-dose continuous treatment group E2 of Yinma Jiedu Granules),with 10 mice in each group.The drug toxicity,alveolar lavage fluid cell count,inflammatory factors and hematoxylin-eosin(HE)staining of lung tissue in each group were compared.Results During the modeling process,a total of 6 mice died,with a success rate of 92.11%.Compared with the control group,the number of inflammatory cells,inflammatory factors,and HE staining infiltration rate of inflammatory cells in the alveolar lavage fluid of the model group were all increased(P<0.05).Compared with normal lung tissue,HE staining of lung tissue in acute lung injury model mice showed alveolar septal congestion,edema,and infiltration of a large number of inflammatory cells.The cell count and inflammatory cytokine levels in the alveolar lavage fluid of successfully modeled mice were higher than those in Group A,and the differences were statistically significant(P<0.05).The state of the model group mice was poor,characterized by towering and clumping hair,significantly reduced food and water intake,reduced body weight,and dry stools.After administration of Yinma Jiedu Granules orally for 1 day to each group of acute lung injury model mice,the C2,D2,and E2 groups of mice were in good condition.After 8 days

关 键 词：银马解毒颗粒 BALB/c急性肺损伤 炎症细胞 炎症因子 

分 类 号：R965[医药卫生—药理学]