壳聚糖-蒙脱土插层复合物对热应激雄性小鼠生殖功能的影响  

Effect of chitosan-montmorillonite intercalationcompound on reproductive function of male mice under heat stress

作　　者：杨焕琳 高元 郭展辉 谭飞 陈浩然 巨向红[1,2] 雍艳红 YANG Huan-lin;GAO Yuan;GUO Zhan-hui;TAN Fei;CHEN Hao-ran;JU Xiang-hong;YONG Yan-hong

机构地区：[1]广东海洋大学动物医学系,广东湛江524088 [2]广东海洋大学深圳研究院,广东深圳518108

出　　处：《饲料研究》2024年第10期87-92,共6页Feed Research

基　　金：广东海洋大学滨海农业学院大学生创新创业基金。

摘　　要：试验研究了壳聚糖-蒙脱土插层复合物(CS-MMT)对热应激雄性小鼠临床症状、睾丸形态和精子质量的保护作用。选择30只6周龄健康雄性ICR小鼠,随机分为对照组(CON组)、热应激组(HS组)、低剂量CS-MMT组(L组,50 mg/kg CS-MMT)、中剂量CS-MMT组(M组,100 mg/kg CS-MMT)和高剂量CS-MMT组(H组,200 mg/kg CS-MMT),先连续给药7 d后,再热应激处理7 d,采集小鼠组织及精液进行观察。结果显示,与CON组相比,HS组小鼠睾丸组织损伤明显,精子密度显著降低(P<0.05),精子活率和顶体完整性极显著降低(P<0.01),精子畸形率和顶体畸形率极显著升高(P<0.01)。与HS组小鼠相比,CS-MMT组小鼠睾丸组织形态结构明显改善,精子密度增加,精子活率和顶体完整性极显著提高(P<0.01),精子畸形率和顶体畸形率极显著降低(P<0.01),呈剂量依赖性。研究表明,CS-MMT具有缓解热应激引起的雄性小鼠生殖功能损伤的作用,以200 mg/kg的给药量效果较好。The protective effects of chitosan-montmorillonite intercalation complex(CS-MMT)on the clinical symptoms,testicular morphology,and sperm quality of heat-stressed male mice were studied.Thirty 6-week-old male ICR mice were randomly divided into control group(CON group),heat stress group(HS group),low-dose CS-MMT group(L group,50 mg/kg CS-MMT),middle-dose CS-MMT group(M group,100 mg/kg CS-MMT),and high-dose CS-MMT group(H group,200 mg/kg CS-MMT).The results showed that compared with CON group,the testicular tissue damage was obvious in HS group,sperm density was significantly decreased(P<0.05),sperm viability and acrosome integrity were extremely decreased(P<0.01),and the sperm malformation and acrosome malformation rates were extremely increased(P<0.01).Compared with HS group,the testicular tissue morphology and structure of mice in CS-MMT groups were significantly improved,the sperm density was increased,the sperm viability and acrosome integrity were extremely increased(P<0.01),and the sperm malformation and acrosome malformation rates were extremely decreased(P<0.01)in a dose-dependent manner.The study indicates that CS-MMT can alleviate the reproductive function damage induced by heat stress in male mice,and and the dosage of 200 mg/kg is better.

关 键 词：壳聚糖-蒙脱土插层复合物 热应激 精子质量 生殖功能 

分 类 号：S816.7[农业科学—饲料科学]