芥子微波和恒温鼓风烘烤炮制工艺研究  

The Processing Technology of Microwave and Constant Temperature Blast Baking of Jiezi(Sinapis Semen)

作　　者：廖小娟[1] 周宜[1] 谢瑜[1] 邹菊英[1] 刘小丽[1] 王庄 代薇 王竹鑫[1] LIAO Xiaojuan;ZHOU Yi;XIE Yu;ZOU Juying;LIU Xiaoli;WANG Zhuang;DAI Wei;WANG Zhuxin(The Second Affiliated Hospital of Hunan University of Chinese Medicine,Changsha Hunan 410005,China)

机构地区：[1]湖南中医药大学第二附属医院,湖南长沙410005

出　　处：《中医药导报》2024年第6期44-48,共5页Guiding Journal of Traditional Chinese Medicine and Pharmacy

基　　金：2021年度湖南省中医药管理局一般项目(2021053);第七批全国老中医药专家王竹鑫教授学术经验继承项目(国中医药人教函〔2022〕76号)。

摘　　要：目的:优化芥子微波和恒温鼓风烘烤炮制工艺。方法:建立白芥子苷含量测定方法;按照析因设计试验法,通过CRITIC法计算外观性状、芥子碱硫氰酸盐和白芥子苷3个指标的权重系数,以综合评分为指标,考察微波火力、恒温鼓风烘烤温度、加热时间对炮制品的影响,选出2种炮制工艺的最佳炮制品;通过噻唑蓝(MTT)比色法考察最佳炮制品与传统文火清炒炮制品对HaCaT细胞活力的影响。结果:芥子微波大火加热2 min、150℃恒温鼓风烘烤30 min得到的炒芥子综合评分最高,芥子碱硫氰酸盐含量符合2020年版《中华人民共和国药典》要求;与传统炮制品比较,白芥子苷含量更高,在800μg/mL浓度时对HaCaT细胞均无明显毒性。结论:微波、恒温鼓风烘烤芥子炮制工艺合理、可行、易操作,可为炒芥子的规范化生产提供参考依据。Objective:To optimize the microwave and constant temperature blast baking process of Jiezi(Sinapis Semen).Methods:The method was developed for the determination of sinalbin in the Jiezi(Sinapis Semen).According to the factorial design test method,weight coefficients of appearance traits,sinapine thiocyanate and sinalbin contents were calculated by CRITIC method.The comprehensive score was used as the index,and the influence of microwave power,constant temperature blast baking temperature and drying time on the quality of processed products were investigated.The best processed products of the two processing technologies were selected.The effects of the best processed products and the traditional slow fire stir fried processed products on the viability of HaCaT cells were investigated by MTT colorimetry.Results:The mustards obtained by microwave high fire heated for 2 min and constant temperature blast baked at 150℃for 30 min got the highest scores respectively,and the content of sinapinethiocyanate met the requirements of the Pharmacopoeia of the People's Republic of China 2020 Edition.Compared with traditional processed products,the sinalbin was higher and there were no obvious toxicity to HaCaT cells at a concentration of 800μg/mL.Conclusion:The processing technology of Jiezi(Sinapis Semen)baked by microwave and constant temperature blast is reasonable,feasible and easy to operate,so as to provide a theoretical reference basis for the standardized production of stir fried Jiezi(Sinapis Semen).

关 键 词：芥子 芥子碱硫氰酸盐 白芥子苷 炮制工艺 HACAT细胞 

分 类 号：R283.1[医药卫生—中药学]