Exposure to ephedrine attenuates Th1/Th2 imbalance underlying OVA-induced asthma through airway epithelial cell-derived exo-somal lnc-TRPM2-AS  

作　　者：HU Yan WANG Mengqing XIE Jing JIAO Luojia DING Yi LUO Yinhe 

机构地区：[1]Department of Pediatrics,The First Hospital of Hunan University of Chinese Medicine,Changsha 410007,China [2]Hunan University of Chinese Medicine,Changsha 410208,China [3]Department of Rehabilitation,Medical College,Changsha Civil Affairs Vocational and Technical College,Changsha 410004,Hunan Province,China [4]Teaching and Research Office of Chinese and Western Combination,Hunan University of Chinese Medicine,Changsha 410208,China

出　　处：《Chinese Journal of Natural Medicines》2024年第6期530-540,共11页中国天然药物（英文版）

基　　金：supported by The Scientific Research Project of Traditional Chinese Medicine in Hunan Province(No.A2024027);The National Inheritance Studio of Distinguished Veteran TCM Experts(Letter of National Traditional Chinese Medicine Education[2022]No.75);Natural Science Foundation of Hunan Province(Nos.2021JJ40422,2023JJ60260).

摘　　要：Although various anti-inflammatory medications,such as ephedrine,are employed to manage cough-variant asthma,their underlying mechanisms are yet to be fully understood.Recent studies suggest that exosomes derived from airway epithelial cells(AECs)contain components like messenger RNAs(mRNAs),micro-RNAs(miRNAs),and long noncoding RNA(lncRNA),which play roles in the occurrence and progression of airway inflammation.This study investigates the influence of AEC-derived exosomes on the efficacy of ephedrine in treating cough-variant asthma.We established a mouse model of asthma and measured airway resist-ance and serum inflammatory cell levels.Real-time polymerase chain reaction(RT-qPCR),Western blotting,and enzyme-linked im-munosorbent assay(ELISA)analyses were used to assess gene and protein expression levels.Exosomes were isolated and character-ized.RNA immunoprecipitation(RIP)and RNA pull-down assays were conducted to examine the interaction between hnRNPA2B1 and lnc-TRPM2-AS1.In the ovalbumin(OVA)-challenged mouse model,ephedrine treatment reduced inflammatory responses,air-way resistance,and Th1/Th2 cell imbalance.Exosomes from OVA-treated AECs showed elevated levels of lnc-TRPM2-AS1,which were diminished following ephedrine treatment.The exosomal lnc-TRPM2-AS1 mediated the Th1/Th2 imbalance in CD4^(+)T cells,with its packaging into exosomes being facilitated by hnRNPA2B1.This study unveils a novel mechanism by which ephedrine ameli-orates OVA-induced CD4^(+)T cell imbalance by suppressing AEC-derived exosomal lnc-TRPM2-AS1.These findings could provide a theoretical framework for using ephedrine in asthma treatment.

关 键 词：ASTHMA EPHEDRINE Exosomal lnc-TRPM2-AS1 Th1/Th2 imbalance HnRNPA2B1 Airway epithelial cells 

分 类 号：R965[医药卫生—药理学]