Anti-inflammatory germacrane-type sesquiterpene lactones from Vernonia sylvatica  

作　　者：WANG Min LI Han HU Bintao TANG Chunping XU Hui KE Changqiang XIE Zuoquan YE Yang YAO Sheng 

机构地区：[1]State Key Laboratory of Drug Research&Natural Products Research Center,Shanghai Institute of Materia Medica,Chinese Academy of Sciences,Shanghai 201203,China [2]University of Chinese Academy of Sciences,Beijing 100049,China [3]School of Life Science and Technology,Shanghai Tech University,Shanghai 201210,China [4]State Key Laboratory of Drug Research,Shanghai Institute of Materia Medica,Chinese Academy of Sciences,Shanghai 201203,China [5]Zhongshan Institute for Drug Discovery,Shanghai Institute of Materia Medica,Chinese Academy of Sciences,Zhongshan 528400,China

出　　处：《Chinese Journal of Natural Medicines》2024年第6期568-576,共9页中国天然药物（英文版）

摘　　要：Nine new germacranolides,sylvaticalides A−H(1-9),and three known analogues(10-12)were isolated from the aeri-al part of Vernonia sylvatica.Their structures were established using comprehensive spectroscopic analysis,including high-resolution electrospray ionization mass spectroscopy(HR-ESI-MS)and 1D and 2D nuclear magnetic resonance(NMR)spectra.Their absolute configurations were determined by X-ray diffraction experiments.The anti-inflammatory activities of all isolated compounds were as-sessed by evaluating their inhibitory effects on the nuclear factor kappa B(NF-κB)pathway,which was activated by lipopolysacchar-ide(LPS)-stimulated human THP1-Dual cells,and the interferon-stimulated gene(ISG)pathway,activated by STING agonist MSA-2 in the same cell model.Compounds 1,2 and 6 showed inhibitory effects on the NF-κB and ISG signaling pathways,with IC_(50)values ranging from 4.12 to 10.57μmol·L^(−1).

关 键 词：Vernonia sylvatica Germacrane-type sesquiterpene lactone Sylvaticalides A−H ANTI-INFLAMMATORY NF-κB path-way ISG pathway 

分 类 号：R284.1[医药卫生—中药学]