2023年青海省玉树市小型啮齿类动物棘球属绦虫感染调查  

Prevalence of Echinococcus infections in small rodents in Yushu City,Qing-hai Province in 2023

作　　者：贺耿城 吴树声 秦香兰 马吉龙 余庭俊 宋成玺 莫筱瑾 马霄[4] 巴建峰 郑贵荣 姜斌 田添 杨诗杰 张颋 周晓农 HE Gengcheng;WU Shusheng;QIN Xianglan;MA Jilong;YU Tingjun;SONG Chengxi;MO Xiaojin;MA Xiao;BA Jian-feng;ZHENG Guirong;JIANG Bin;TIAN Tian;YANG Shijie;ZHANG Ting;ZHOU Xiaonong(National Institute of Parasitic Diseases,Chinese Center for Disease Control and Prevention(Chinese Center for Tropical Diseases Research),National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases,National Health Commission Key Laboratory of Parasite and Vector Biology,WHO Collaborating Centre for Tropical Diseases,National Center for International Research on Tropical Diseases,Ministry of Science and Technology,Shanghai 200025,China;Yushu Tibetan Autonomous Prefecture Center for Disease Control and Prevention,Qinghai Province,China;Tibet Autonomous Region Center for Disease Control and Prevention,National Health Commission Key Laboratory of Echinococcosis Prevention and Control,China;Qinghai Institute for Endemic Disease Control and Prevention,China;Shiqu County Center for Disease Control and Prevention,Sichuan Province,China;School of Life Sciences,Inner Mongolia University,China)

机构地区：[1]中国疾病预防控制中心寄生虫病预防控制所(国家热带病研究中心)、传染病溯源预警与智能决策全国重点实验室、国家卫生健康委员会寄生虫病原与媒介生物学重点实验室、WHO热带病合作中心、科技部国家级热带病国际联合研究中心,上海200025 [2]青海省玉树藏族自治州疾病预防控制中心 [3]西藏自治区疾病预防控制中心、国家卫生健康委包虫病防治研究重点实验室 [4]青海省地方病预防控制所 [5]四川省石渠县疾病预防控制中心 [6]内蒙古大学生命科学学院

出　　处：《中国血吸虫病防治杂志》2024年第2期169-173,共5页Chinese Journal of Schistosomiasis Control

基　　金：国家重点研发计划(2021YFC2300800,2021YFC2300804);国家卫生健康委包虫病防治研究重点实验室开放课题(2021WZK1003)。

摘　　要：目的 了解青海省玉树市人群聚居区周边小型啮齿类动物棘球属绦虫感染率,为进一步开展棘球蚴病精准防控提供参考。方法 2023年6月,于青海省玉树市上拉秀乡与隆宝镇牧场分别随机设置1~2处50 m×50 m样方,每个样方内放置300个板式鼠夹(12.0 cm×6.5 cm),每日10:00—18:00捕捉小型啮齿类动物,持续4 d。对捕获的啮齿类动物进行鉴定、剖检,对疑似棘球蚴感染的肝脏组织进行病理学检查。采用PCR法扩增棘球属绦虫细胞色素c氧化酶1(cytochrome c oxidase 1,cox1)基因,并与Gen Bank数据库中的序列进行比对以鉴定虫种,以邻接法构建棘球绦虫系统进化树。结果 在玉树市上拉秀乡与隆宝镇累计捕获小型啮齿类动物236只,其中上拉秀乡捕获青海田鼠65只(占56.0%)、高原鼠兔51只(占44.0%);隆宝镇捕获青海田鼠62只(占51.7%)、高原鼠兔58只(占48.3%),两地小型啮齿类动物构成差异无统计学意义(χ^(2)=0.294,P> 0.05)。剖检共发现7只高原鼠兔和12只青海田鼠疑似感染棘球蚴,病理学检查可见疑似棘球蚴感染动物肝脏肝小叶结构不清、肝细胞排列紊乱。PCR扩增疑似棘球蚴感染病灶组织DNA,发现7只青海田鼠感染石渠棘球蚴,均捕获自上拉秀乡。系统进化分析发现,捕获的小型啮齿类动物所感染棘球蚴cox1基因序列与已报道的石渠棘球绦虫cox1基因序列高度同源。结论 2023年青海省玉树市人群聚居区周边小型啮齿类动物以高原鼠兔和青海田鼠为主,存在石渠棘球蚴感染。Objective To investigate the prevalence of Echinococcus infections in small rodents around human residential areas in Yushu City,Qinghai Province in 2023,so as to provide insights into precision echinococcosis control.Methods One or two quadrats,each measuring 50 m×50 m,were randomly assigned in Shanglaxiu Township and Longbao Township,Yushu City,Qinghai Province on June 2023,respectively,and 300 plate-type mouse traps,each measuring 12.0 cm×6.5 cm,were assigned in each quadrat.Small rodents were captured during the period between 10:00 and 18:00 each day for 4 days.Then,all captured small rodents were identified and dissected,and liver specimens with suspected Echinococcus infections were subjected to pathological examinations.The Echinococcus cytochrome c oxidase 1(cox1)gene was amplified using PCR assay,and the sequence of the amplified product was aligned to that was recorded in the GenBank to characterize the parasite species.In addition,a phylogenetic tree of Echinococcus was generated based on the cox1 gene sequence using the neighbor-joining method.Results A total of 236 small rodents were captured in Shanglaxiu and Longbao townships,Yushu City,including 65 Qinghai voles and 51 plateau pikas in Shanglaxiu Township,and 62 Qinghai voles and 58 plateau pikas in Longbao Township,and there was no significant difference in the constituent ratio of small rodents between the two townships(χ^(2)=0.294,P>0.05).Seven plateau pikas and 12 Qinghai voles were suspected to be infected with Echinococcus by dissection,and pathological examinations showed unclear structure of hepatic lobules and disordered hepatocyte arrangement in livers of small rodents suspected of Echinococcus infections.PCR assay identified E.shiquicus DNA in 7 Qinghai voles,which were all captured from Shanglaxiu Township.Phylogenetic analysis showed that the cox1 gene sequence of Echinococcus in small rodents was highly homologous to the E.shiquicus cox1 gene sequence reported previously.Conclusion Plateau pika and Qinghai vole were predominant s

关 键 词：棘球蚴病 石渠棘球绦虫 高原鼠兔 青海田鼠 细胞色素c氧化酶1 玉树市 

分 类 号：R383.33[医药卫生—医学寄生虫学]