假病毒包装系统发展及新兴应用  

Development and Emerging Applications of Packaging Systems for Pseudoviruses

作　　者：葛慧敏 蒋雯玲 方中岳 李少伟[1,2] 顾颖[1,2] GE Huimin;JIANG Wenling;FANG Zhongyue;LI Shaowei;GU Ying(National Institute of Diagnostics and Vaccine Development in Infection Diseases,State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics,School of Public Health,Xiamen University,Xiamen 361102,China;School of Life Sciences,Xiamen University,Xiamen 361102,China)

机构地区：[1]厦门大学国家传染病诊断试剂与疫苗工程技术研究中心,分子疫苗学和分子诊断学国家重点实验室,公共卫生学院,厦门361102 [2]厦门大学生命科学学院,厦门361102

出　　处：《病毒学报》2024年第3期631-640,共10页Chinese Journal of Virology

基　　金：国家自然科学基金(项目号:82171821),题目:人免疫缺陷病毒新型免疫原的分子设计及其广谱中和免疫原性的研究。

摘　　要：假病毒是一种人工制造的嵌合重组病毒颗粒。根据研究需要,可基于不同病毒载体包装系统,使病毒颗粒表面携带其它病毒的包膜蛋白/衣壳蛋白。重组假病毒颗粒具有与活病毒相似的结构及功能,可作为活病毒的替代品用于病毒学研究。本文综述了基于不同病毒载体包装系统的常用假病毒构建策略、影响假病毒构建/滴度的因素及假病毒的新兴应用,以期为高致病性、高传染性病毒的安全研究提供参考思路。Pseudovirus is an artificially produced chimeric recombinant virus particle.According to research needs,different“packaging systems”for viral vectors can be used to carry the envelope proteins/capsid proteins of other viruses on the surface of virus particles.Recombinant pseudovirions have similar structures and functions to live viruses,and can be used as substitutes for live viruses in virology research.This article reviews common construction strategies for pseudoviruses based on different packaging systems for viral vectors,the factors affecting pseudovirus construction/titer,and emerging applications of pseudoviruses.This article provides reference ideas for biosafety research on highly pathogenic and highly infectious viruses.

关 键 词：假病毒 包装系统 影响因素 新兴应用 

分 类 号：R373.9[医药卫生—病原生物学]