基于近红外光谱分析技术的菊花品种识别及3种质量标志物含量快速测定  

Identification of Chrysanthemum Varieties and Rapid Determination of Three Quality Markers Based on Near-Infrared Spectroscopy

作　　者：彭璐 吴卫刚 范荫荫 薄雯映 李文龙 徐凌峰 梅之南 PENG Lu;WU Wei-gang;FAN Yin-yin;BO Wen-yin;LI Wen-Long;XU Ling-Feng;MEI Zhi-Nan(Hebei Chufeng Traditional Chinese Medicine Pieces Co.,Ltd,Baoding 071200,China;Tianjin University of Traditional Chinese Medicine,Tianjin 301617,China;Huazhong Agricultural University,Wuhan 430070,China)

机构地区：[1]河北楚风中药饮片有限公司,河北保定071200 [2]天津中医药大学,天津301617 [3]华中农业大学,湖北武汉430070

出　　处：《时珍国医国药》2024年第4期898-901,共4页Lishizhen Medicine and Materia Medica Research

基　　金：河北省创新能力提升计划项目(215A2501D);湖北省科技重大专项(2021ACA004)。

摘　　要：目的建立能快速识别不同品种菊花及快速测定其3种质量标志物的方法。方法分别采用偏最小二乘法和正交偏最小二乘判别分析法建立菊花中绿原酸、木犀草苷和3,5-O-二咖啡酰基奎宁酸的定量分析模型以及菊花品种识别的定性分析模型。结果所构建的定量分析模型对校正集中3种质量标志物进行预测,相关系数(R)依次为0.9215,0.9404,0.9795,校正均方差(RMSEC)依次为0.0880%,0.0522%,0.1150%,预测均方差(RMSEP)依次为0.0911%,0.0452%,0.1760%。经统计学分析,定量分析模型对验证集的预测值与参考值无显著性差异。O2PLS-DA模型可以快速区分不同品种菊花。结论近红外光谱技术结合化学计量学方法可以实现对菊花3种质量标志物含量的快速测定和不同品种菊花快速识别。Objective To establish a method for the rapid identification of different varieties of Chrysanthemum and the rapid determination of three quality markers.Methods The quantitative analysis models for chlorogenic acid,luteolin and 3,5-O-dicaffeoylquinic acid in Chrysanthemum were established by partial least squares(PLS),and the qualitative analysis model for the identification of Chrysanthemum varieties was established by orthogonal partial least squares discriminant analysis(O2PLS-DA).Results The constructed quantitative analysis model predicted the three quality markers in the calibration set with correlation coefficients(R)of 0.9215,0.9404 and 0.9795,root mean square error of calibration(RMSEC)of 0.0880%,0.0522%and 0.1150%,and relative standard error of prediction(RMSEP)of 0.0911%,0.0452%and 0.1760%,respectively.Statistical analysis showed no significant difference between the predicted values of the quantitative analysis models for the validation set and the reference values.The O2PLS-DA model can quickly distinguish different varieties of Chrysanthemum.Conclusion Near-infrared spectroscopy combined with chemometrics can achieve the rapid determination of three quality markers in Chrysanthemum and the rapid identification of different varieties of Chrysanthemum.

关 键 词：近红外光谱技术 菊花 偏最小二乘法 正交偏最小二乘判别分析 质量评价 

分 类 号：R282.5[医药卫生—中药学]