推拿对实验性结肠炎小鼠抗炎作用的蛋白质组学研究  

Proteomic study of Tuina on the colon of the experimental colitis mice

作　　者：王厚融 王湘宜 张英琦[1] 于天源[1] 鲁梦倩[1] WANG Hou-rong;WANG Xiang-yi;ZHANG Ying-qi;YU Tian-yuan;LU Meng-qian(Beijing University of Chinese Medicine,Bejing 102488,China;Acupuncture and Massage Department,Beijing Hospital,National Center of Gerontology,Institute of Geriatric Medicine,Chinese Academy of Medical Sciences,Beijing 100730,China)

机构地区：[1]北京中医药大学,北京102488 [2]北京医院针灸按摩科、国家老年医学中心、中国医学科学院老年医学研究院,北京100730

出　　处：《时珍国医国药》2024年第4期1009-1012,共4页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(82374612);北京中医药大学大学生创新创业训练计划(X202210026017)。

摘　　要：目的探究推拿对实验性结肠炎小鼠的治疗作用及机制。方法将18只雄性BALA/c小鼠随机分为空白组、模型组和推拿组。模型组和推拿组通过葡聚糖硫酸钠(DSS)诱导实验性结肠炎模型。推拿组在造模后7 d进行干预,实验过程中记录体重、血便等一般情况,干预7次后,取结肠组织进行HE染色和蛋白质组学研究。结果推拿干预后UC小鼠一般情况改善,HE染色显示结肠组织病变程度减轻。蛋白质组学结果显示与推拿作用相关的差异蛋白共有216个,iPath分析得到402条代谢途径,KEGG富集分析到262条通路。结论推拿能够改善UC炎症表现,其作用机制可能与维生素消化吸收、花生四烯酸代谢、TRP通道的炎症介质调节有关。Objective To explore the therapeutic effect and mechanism of Tuina on experimental colitis mice.Methods Eighteen male BALA/c mice were randomly divided into the normal,model,and tuina groups.The experimental colitis model was induced by dextran sodium sulfate(DSS)in the model and Tuina group.The Tuina group was intervened 7 d after modeling,and the general conditions such as body weight and blood stool were recorded during the experiment.After 7 interventions,colon tissues were taken for HE staining and proteomics study.Results The general condition of UC mice improved after Tuina interventions,and HE staining showed a reduced degree of colon tissue pathologies.The proteomic results showed a total of 216 differential proteins associated with the effect of Tuina,402 metabolic pathways identified by iPath analysis,and 262 pathways enriched by KEGG analysis.Conclusion China can improve the inflammatory symptoms of UC,and its action mechanism may be related to vitamin digestion and absorption,arachidonic acid metabolism,and regulation of inflammatory mediators by TRP channels.

关 键 词：推拿 摩腹法 溃疡性结肠炎 蛋白组学 结肠 花生四烯酸 瞬时感受器电位通道 

分 类 号：R2-03[医药卫生—中医学]