miRNA表达谱和靶基因分析在糖尿病轻瘫发病机制中的作用  

Exploring molecular mechanisms of diabetic gastroparesis:Insights from miRNA expression profiles and target gene analysis

作　　者：孙嘉咛 张默函 SUN Jianing;ZHANG Mohan(Department of Clinical Medicine,College of Medicine,Yanbian University,Yanji,Jilin 130oo0,China;Department of Histology and Embryology,College of Medicine,Yanbian University,Yanji,Jilin 130oo0,China)

机构地区：[1]延边大学医学院临床医学系,吉林延吉133000 [2]延边大学医学院组织学与胚胎学教研室,吉林延吉133000

出　　处：《中国实验诊断学》2024年第6期714-721,共8页Chinese Journal of Laboratory Diagnosis

基　　金：国家自然科学基金项目(82060154);吉林省自然科学基金项目(YDZJ202201ZYTS147)。

摘　　要：目的本研究旨在通过分析微小RNA(miRNAs)在糖尿病胃轻瘫(Diabetic Gastroparesis,DGP)中的表达变化,揭示其在疾病发病机制中的潜在作用。方法通过基因表达综合数据库(Gene Expression Omnibus,GEO)获取糖尿病胃轻瘫患者和健康对照者的血浆miRNA测序数据,使用limma软件包鉴定差异表达miRNAs,并预测其靶基因。结合Gene Cards数据库获取糖尿病胃轻瘫的潜在靶点,进行功能富集分析和蛋白质-蛋白质相互作用网络构建。结果研究共筛选出43个在糖尿病胃轻瘫患者中差异表达的miRNAs,并预测了其对应的171个靶基因。功能富集分析显示这些靶基因涉及多种生物学过程、细胞组分和分子功能,与糖尿病胃轻瘫的发病机制密切相关。蛋白质-蛋白质相互作用网络构建和枢纽基因筛选揭示了一批关键基因,如PRKN、SNCA、PINK1等,可能在疾病发展中发挥重要调控作用。结论本研究通过深入探索miRNAs在糖尿病胃轻瘫中的表达变化及其靶基因情况,揭示了该疾病的潜在分子机制。发现的关键基因可能成为治疗靶点,为糖尿病胃轻瘫的治疗提供新的思路和策略。Objective This study aims to elucidate the potential role of microRNAs(miRNAs) in the pathogenesis of diabetic gastroparesis(DGP) by analyzing their expression changes in the disease.Methods Plasma miRNA sequencing data of patients with diabetic gastroparesis and healthy controls were obtained from the Gene Expression Omnibus(GEO) database.Differential expression miRNAs were identified using the limma package, and their target genes were predicted.Functional enrichment analysis and protein-protein interaction network construction were performed by integrating potential DGP target genes from the Gene Cards database.Results A total of 43 differentially expressed miRNAs in patients with diabetic gastroparesis were identified, with 171 corresponding target genes predicted.Functional enrichment analysis revealed the involvement of these target genes in various biological processes, cellular components, and molecular functions closely related to the pathogenesis of diabetic gastroparesis.Protein-protein interaction network construction and hub gene screening revealed a set of key genes, such as PRKN,SNCA,and PINK1,which may play crucial regulatory roles in disease progression.Conclusion This study provides insights into the potential molecular mechanisms of diabetic gastroparesis by investigating the expression changes of miRNAs and their target genes.The identified key genes may serve as therapeutic targets, offering new insights and strategies for the treatment of diabetic gastroparesis.

关 键 词：糖尿病胃轻瘫 微小RNA 靶基因 蛋白质-蛋白质相互作用 治疗靶点 

分 类 号：R587.1[医药卫生—内分泌]