玉米花粉离体萌发的研究  

Study on Maize Pollen Germination in vitro

作　　者：范明霞 杨雅婷 苏立新 杨双[2] 王贺 高松 FAN Mingxia;YANG Yating;SU Lixin;YANG Shuang;WANG He;GAO Song(College of Life Science and Engineering,Shenyang University,Shenyang 110044;Shenyang Academy of Agricultural Sciences,Shenyang 110161;Shenyang Rural Revitalization and Development Center,Shenyang 110121)

机构地区：[1]沈阳大学生命科学与工程学院,沈阳110044 [2]沈阳市农业科学院,沈阳110161 [3]沈阳市乡村振兴发展中心,沈阳110121

出　　处：《中国农学通报》2024年第17期21-27,共7页Chinese Agricultural Science Bulletin

基　　金：国家自然基金“高温胁迫下类受体蛋白激酶ZmSTK1和ZmSTK2通过糖酵解代谢调控玉米花粉育性的机制”(32001462)。

摘　　要：本研究旨在探究不同培养基组分对玉米花粉萌发的影响,并筛选出最适宜玉米花粉离体萌发的培养基。通过单因素实验,比较了不同浓度的蔗糖、葡萄糖、乳糖、麦芽糖、硼酸和氯化钙对多个玉米品种花粉活力的作用。结果表明,含有0.35 mol/L蔗糖、0.85%琼脂、0.01%硼酸和0.03%氯化钙的培养基能够使对照花粉达到最佳萌发效果,为51.6%。然而,该培养基对于不同品种的玉米花粉影响差异显著,实验中8个玉米品种的花粉萌发率在1.8%~21%之间,显示出‘e12’品种的花粉萌发最为适宜,而‘e11’品种的花粉萌发则不适宜。本研究筛选出的最适培养条件为玉米花粉离体萌发机制的研究及玉米遗传育种提供了重要的理论依据和参考。The purpose of this study was to investigate the effect of different culture medium components on the germination of maize pollen,in order to screen out the optimal culture medium for in vitro germination of maize pollen,and further select suitable culture media for pollen germination of different maize varieties.In this experiment,in vitro germination method was used to test different maize varieties.Single-factor experiments were conducted to compare the effects of different concentrations of sucrose,glucose,lactose,maltose,boric acid,and calcium chloride on the vitality of maize pollen,and to explore the optimal conditions for maize pollen germination.The results showed that when the culture medium components were 0.35 mol/L sucrose,0.85% agar,0.01% boric acid,and 0.03% calcium chloride,the germination effect of the control group was the best,reaching 51.6%.Compared with the control group,the experimental group found that this medium had significant differences in the germination of pollen of different varieties.In this study,the pollen germination rates of 8 experimental groups of maize varieties ranged from 1.8%to 21%,and this medium was most suitable for the germination of pollen of variety‘e12’,but not suitable for the germination of variety‘e11’.In conclusion,the optimal culture conditions conducive to in vitro germination of maize pollen were screened out,providing valuable reference and theoretical basis for the study of maize pollen germination mechanism,maize genetic breeding,and other related research areas.

关 键 词：玉米花粉 花粉活力 培养基 离体培养 培养条件 萌发率 萌发 单因素实验 品种差异 

分 类 号：S513[农业科学—作物学]