132份甘薯地方种质遗传多样性和群体结构分析  

132 Sweetpotato Landraces:Genetic Diversity and Population Structure

作　　者：陈伊航 唐朝臣 张荣 姚祝芳 金晶炜[1] 王章英[2] CHEN Yihang;TANG Chaochen;ZHANG Rong;YAO Zhufang;JIN Jingwei;WANG Zhangying(College of Grassland Agriculture,Northwest A&F University,Yangling,Shaanxi 712100;Crops Research Institute,Guangdong Academy of Agricultural Sciences/Guangdong Provincial Key Laboratory of Crop Genetic Improvement,Guangzhou 510640)

机构地区：[1]西北农林科技大学草业与草原学院,陕西杨陵712100 [2]广东省农业科学院作物研究所/广东省农作物遗传改良重点实验室,广州510640

出　　处：《中国农学通报》2024年第18期22-30,共9页Chinese Agricultural Science Bulletin

基　　金：作物种质资源保护与利用专项“甘薯种质资源安全保存”(NWB014);国家作物种质资源库“甘薯广州分库运行分库”(NCGRC-2023-39);现代农业产业技术体系“国家甘薯产业技术体系”(CARS-10)。

摘　　要：本研究旨在评估中国甘薯地方种质资源的遗传多样性和群体结构。研究以132份甘薯地方种质为材料,对其表型性状、类胡萝卜素含量以及SSR分子标记进行遗传多样性分析和群体结构分析。分析结果表明,132份甘薯地方种质表型性状的平均变异系数为45.84%,类胡萝卜素含量平均为6.33μg/g,最高为59.90μg/g,SSR分子标记的Nei’s多样性指数平均为0.15、Shannon’s多样性指数平均为0.23,表明存在一定程度的遗传多态性。进一步的群体结构分析表明,这132份甘薯地方种质可分为5个亚群。分子变异分析(AMOVA)显示,84%的变异存在于群体内,16%的变异存在于群体间。综上所述,中国的甘薯地方种质具有丰富的遗传多样性,且种质之间的遗传变异主要集中在亚群内部。这对于中国甘薯地方种资源的保护和新品种的培育提供了重要的信息。Understanding the genetic diversity and population structure of sweetpotato landraces in China is helpful for the protection of sweetpotato germplasm resources and the cultivation of new varieties.In this study,132 sweet potato landraces were used as materials to analyze the genetic diversity and population structure of phenotypic traits,carotenoid content,and SSR molecular markers.The results showed that the average coefficient of variation of phenotypic traits of 132 sweetpotato landraces was 45.84%,the average carotenoid content was 6.33μg/g,and the highest was 59.90μg/g.The average Nei's diversity index of SSR molecular markers was 0.15,and the average Shannon's diversity index was 0.23.Based on the population structure analysis,the 132 sweetpotato landraces were divided into five subpopulations.Further analysis of molecular variation revealed that 84%of the variation existed within populations and 16%between populations.In summary,sweetpotato landraces have rich genetic diversity,and the genetic variation among germplasms is mainly concentrated within the subpopulation.This provides important information for the protection of local sweetpotato germplasm resources and the cultivation of new varieties in China.

关 键 词：甘薯 表型性状 SSR分子标记 类胡萝卜素含量 遗传多样性 群体结构 地方种质 

分 类 号：S324[农业科学—作物遗传育种]