外源褪黑素对旱胁迫下花生种子萌发及抗性的影响  

Effect of Exogenous Melatonin on Germination and Resistance Ability of Peanut Seeds Under Drought Stress

作　　者：孙赛楠 苗鹏慧 权月伟[2] 穆国俊[1] 郭丽果[1] 杨鑫雷[1] SUN Sainan;MIAO Penghui;QUAN Yuewei;MU Guojun;GUO Liguo;YANG Xinlei(State Key Laboratory of North China for Crop Improvement and Regulation/North China Key Laboratory for Crop Germplasm Resources,Ministry of Education/Laboratory of Crop Germplasm Resources of Hebei Province/College of Agronomy,Hebei Agricultural University,Baoding,Hebei 071001;Handan Academy of Agricultural Sciences,Handan,Hebei 056000)

机构地区：[1]华北作物改良与调控国家重点实验室/华北作物种质资源教育部重点实验室/河北省种质资源实验室/河北农业大学农学院,河北保定071001 [2]邯郸市农业科学院,河北邯郸056000

出　　处：《中国农学通报》2024年第18期38-44,共7页Chinese Agricultural Science Bulletin

基　　金：国家自然科学基金项目“花生侧枝角度性状的遗传分析和QTL定位”(31701459);河北省青年拔尖人才资助项目(0602015);河北省高等学校科学技术研究项目“基于Meta和连锁分析挖掘花生荚果大小QTL及候选基因”(ZD2022069);河北农业大学科研发展基金计划项目“花生荚果大小相关性状QTL定位”(JY2021019);华北作物改良与调控国家重点实验室自主课题项目“基于Meta和连锁分析挖掘花生产量性状QTL及候选基因”(NCCIR2022zz-6)。

摘　　要：本研究旨在探索外源褪黑素对干旱胁迫下花生种子萌发及抗性的影响。以干旱敏感型花生品种‘冀花5号’为试验材料,通过筛选种子萌发其适宜的PEG和褪黑素浓度,并设置空白水浸种(CK),褪黑素浸种(T_(1)),10%PEG-6000处理(T_(2))和褪黑素浸种加10%PEG-6000处理(T_(3))等4种处理,于种子萌发后进行抗氧化生理生化指标的测定。研究结果显示,模拟干旱的PEG-6000浓度为10%时,可以模拟干旱环境。同时,100µmol/L的褪黑素浓度是促进花生种子萌发的最佳选择。进一步分析各处理花生种子胚根长度与粗度和抗氧化生理生化指标,发现干旱胁迫下施用100µmol/L的褪黑素处理显著增强胚根生长势,并显著提高种子内SOD、POD和Pro的含量,且有效降低种子内MDA和H_(2)O_(2)的含量。综上所述,本研究表明外源褪黑素可以有效提高干旱胁迫下花生种子的萌发,为进一步探究干旱胁迫下褪黑素促进花生种子萌发的调控机制提供了理论依据。The study aimed to explore the effect of exogenous melatonin on seed germination and resistance of peanut under drought stress.In this study,the drought-sensitive peanut variety‘Jihua 5’was used as the test material,and four treatments were set up,including blank water-soaked seed(CK),melatonin-soaked seed(T1),10%PEG-6000 treatment(T2),and melatonin-soaked seed plus 10%PEG-6000 treatment(T3),and four other treatments were used to determine the antioxidant physiological and biochemical indices after seed germination.The results showed that the 10%PEG-6000 was effective in simulating the drought environment.Meanwhile,melatonin concentration of 100µmol/L was the best choice to promote peanut seed germination.Further analyzing the length and thickness of radicle and antioxidant physiological and biochemical indexes of peanut seeds under each treatment,we found that the application of 100µmol/L melatonin under drought stress significantly enhanced the growth potential of radicle and increased the contents of SOD,POD and Pro,and effectively reduced the contents of MDA and H_(2)O_(2) in peanut seeds.This study indicated that exogenous melatonin could effectively improve the germination of peanut seeds under drought stress,and provided a theoretical basis for further investigation of the regulatory mechanism of melatonin in promoting peanut seed germination under drought stress.

关 键 词：花生种子 褪黑素 干旱胁迫 种子萌发 外源褪黑素 抗性 抗氧化生理生化指标 

分 类 号：S565.2[农业科学—作物学]