水稻香型抗病恢复系的分子鉴定及其应用  

Rice Restorer Line with Fragrance and Disease Resistance:Molecular Identification and Application

作　　者：张玉婷 王泓超 梁莹莹 匡梦鑫 吴欣 蒋云林 程祖锌[1,2] 林荔辉 ZHANG Yuting;WANG Hongchao;LIANG Yingying;KUANG Mengxin;WU Xin;JIANG Yunlin;CHENG Zuxin;LIN Lihui(Key Laboratory of Genetics,Breeding and Multiple Utilization of Crops,Ministry of Education,Fujian Agriculture and Forestry University,Fuzhou 350002;Fujian Engineering Technology Research Center of Breeding and Utilization for Special Crops,Fuzhou 350002)

机构地区：[1]福建农林大学作物遗传育种与综合利用教育部重点实验室,福州350002 [2]福建省特种作物育种与利用工程技术研究中心,福州350002

出　　处：《中国农学通报》2024年第18期90-95,共6页Chinese Agricultural Science Bulletin

基　　金：福建省科技计划高校产学合作项目“绿色优质专用特种稻新品种的培育及其产业化”(2022N5011);福建省科技厅引导性项目“水稻粳型亲籼型种质资源的创制与利用”(2023N0005)。

摘　　要：提高水稻恢复系的稻米品质及稻瘟病抗性,是培育高产、优质、抗病的杂交稻新组合基础。采用香味fgr基因和稻瘟病抗性基因Pi-1、Pi-9和Pi-kh紧密连锁的分子标记,对香型品种‘玉针香’与抗病恢复系‘福恢683’‘、金恢1131’杂交获得的265个F_(6)代恢复株系进行香味和稻瘟病抗性基因的分子标记辅助选择,筛选出携带上述4个目的基因的6个优良恢复株系,通过综合性状考察,育成了中抗稻瘟病的香型恢复系‘润香’,并配组出高产、优质的香型黑米杂交稻新组合‘紫两优润香’先后通过广西(桂审稻2021200号)和福建省(闽审稻20220024)农作物新品种审定。该品种表现出高产、优质和广适应特性,具有较大的推广应用潜力。本研究表明,分子标记辅助选择是一种在水稻香味和稻瘟病抗性定向改良中的快速而有效的现代生物育种技术。Improving the grain quality and blast resistance of rice restorer lines is the foundation for breeding new hybrid rice combinations with high yield,superior quality and disease resistance.By using molecular markers closely linked to the fragrance gene fgr and rice blast resistance genes Pi-1,Pi-9 and Pi-kh,the molecular marker-assisted selection of fragrance and blast resistance genes was performed in 265 F6 restore line generations derived from aromatic variety‘Yuzhenxiang’crossed with disease resistant restorer lines‘Fuhui 683’and‘Jinhui 1131’.Six excellent restore lines carrying the aforementioned four target genes were selected.Combined with comprehensive character investigation,an aromatic restore line‘Runxiang’with medium resistance to rice blast was bred.And a new combination of high-yield and superior-quality aromatic black hybrid rice‘Ziliangyou Runxiang’had passed the approval of new crop varieties in Guangxi Province(Guishendao NO.2021200)and Fujian Province(Minshendao 20220024),which exhibited high yield,superior grain quality,and great potential for widespread cultivation.Molecular marker-assisted technology is a rapid and effective modern biotechnology breeding technique for the targeted improvement of rice aroma and blast resistance.

关 键 词：水稻 恢复系 稻瘟病 香味 分子鉴定 

分 类 号：S511[农业科学—作物学]