47份野生大豆草甘膦耐性的培养皿鉴定  

Identification of Glyphosate Tolerance of 47 Wild Soybeans Materials in Petri Dishes

作　　者：孙亚萍 胡雪洁 石晓坤 赵圆 郑佳喆 王艳丽 李晓明 乔亚科 秦君[2] 张锴 SUN Yaping;HU Xuejie;SHI Xiaokun;ZHAO Yuan;ZHENG Jiazhe;WANG Yanli;LI Xiaoming;QIAO Yake;QIN Jun;ZHANG Kai(Hebei Key Laboratory of Crop Stress Biology,Hebei Normal University of Science&Technology,Qinhuangdao,066600;Institute of Grain and Oil Crops,Hebei Academy of Agriculture and Forestry Sciences,China)

机构地区：[1]河北科技师范学院农学与生物科技学院,河北省作物逆境生物学重点实验室,河北秦皇岛066600 [2]河北省农林科学院粮油作物研究所

出　　处：《河北科技师范学院学报》2024年第2期1-9,共9页Journal of Hebei Normal University of Science & Technology

基　　金：河北省重点研发计划项目(项目编号:19226365D);河北省大豆产业技术体系(项目编号:326-0702-JSNTKSF);河北省科技厅现代种业科技创新专项大豆现代种业科技创新团队(项目编号:21326313D)。

摘　　要：为确定野生大豆耐草甘膦特性,采用培养皿鉴定法对采集于燕山山脉47份野生大豆材料的芽期耐草甘膦能力进行了鉴定。通过对野生大豆材料芽期发芽势、发芽率、胚轴长、胚根长等指标的测定,结合相关性分析、主成分分析和隶属函数综合评价等方法计算得出野生大豆草甘膦耐性的综合得分。结果表明,8个性状指标转化为2个主成分,累计贡献率达90.43%。将其耐性等级分为4级,其中,野生大豆材料2011编8的草甘膦耐性综合得分最高,2011编42的草甘膦耐性综合得分最低。培养皿鉴定法是一种快速、准确的野生大豆耐草甘膦性状评价方法,可用于野生大豆草甘膦耐性材料的筛选。In order to determine the glyphosate tolerance of wild soybean,the glyphosate tolerance of 47 wild soybean materials collected near Yanshan Mountain was identified by petri dish identification method.By measuring the germination potential,germination rate,cotyl length and radicle length of wild soybean materials,and combining correlation analysis,principal component analysis and membership function evaluation,the comprehensive score of wild soybean glyphosate tolerance was calculated.The results showed that the 8 traits were transformed into 2 principal components,and the cumulative contribution rate was 90.43%.The tolerance level was divided into 4 levels.Glyphosate tolerance of wild soybean materials 2011-8 was the highest and glyphosate tolerance of wild soybean materials 2011-42 was the lowest.The results showed that petri dish identification method was a fast and accurate method to evaluate glyphosate tolerance of wild soybean,and could be used for screening wild soybean glyphosate tolerance materials.

关 键 词：野生大豆 耐性鉴定 耐草甘膦 培养皿鉴定法 

分 类 号：S565.102.4[农业科学—作物学]