玉米茎腐病致病菌全基因组分泌蛋白预测与分析  

Genome-wide Prediction and Analysis of the Secreted Proteins from the Pathogenic Fungus Causing Maize Stalk Rot

作　　者：曹言勇[1] 张立立 李慧敏 韩胜博 侯梦薇 段灿星[2] 施艳[3] 张新友[1] CAO Yan-yong;ZHANG Li-li;LI Hui-min;HAN Sheng-bo;HOU Meng-wei;DUAN Can-xing;SHI Yan;ZHANG Xin-you(Institute of Cereal Crops,Henan Academy of Agricultural Sciences/The Shennong Laboratory,Zhengzhou 450002;Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081;College of Plant Protection,Henan Agricultural University,Zhengzhou 450002,China)

机构地区：[1]河南省农业科学院粮食作物研究所/神农种业实验室,郑州450002 [2]中国农业科学院作物科学研究所,北京100081 [3]河南农业大学植物保护学院,郑州450002

出　　处：《玉米科学》2024年第4期110-118,共9页Journal of Maize Sciences

基　　金：河南省农业科学院自主创新基金项目(2023ZC010);国家重点研发项目(2021YFD1200700)。

摘　　要：九州镰孢菌(Fusarium kyushuense)是一种重要的植物病原菌,可引起玉米茎腐病。为明确九州镰孢菌的分泌蛋白,运用SignalP 6.0、ProtComp 9.0、TMHMM 2.0、big-PI Predictor、TargetP 2.0等软件对九州镰孢菌全基因组12225条蛋白质序列进行分泌蛋白的预测和筛选。结果表明,九州镰孢菌195全基因组蛋白中共有539条序列具有典型的分泌蛋白特征,占总序列数的4.41%。信号肽-3和-1位置的氨基酸相对保守,其切割位点属于A-X-A型。分泌蛋白功能注释分析结果表明,九州镰孢菌的分泌蛋白中共有264个效应蛋白,其功能主要涉及细胞壁组分降解。对碳水化合物活性酶类(CAZymes)进行预测发现,九州镰孢菌分泌蛋白组中共有168个碳水化合物活性酶,为进一步探究其生物学特性、致病机制和筛选新的镰孢菌致病效应蛋白奠定基础。Fusarium kyushuense,one of the causal agents of maize stalk rot.To investigate the mechanism underlying the fungal pathogenicity,secreted proteins of F.kyushuense were predicted by combined using of SignalP 6.0,ProtComp 9.0,TMHMM 2.0,big-PI Predictor and TargetP 2.0 software based on the genome-wide of 12225 protein sequences.In this study,539 secreted proteins were verified,accounting for 4.41%of the total proteins present in the genome of F.kyushuense.In addition,the amino acids in the position-3 to-1 in the signal peptides were relatively conserved and the signal peptide cleavage site belonged to A-X-A type.The effector prediction results indicated that 264 secreted proteins might serve as potential effectors,which mainly involves the degradation of cell wall components.The CAZymes prediction results showed that 168 ones among F.kyushuense putative secreted proteins were carbohydrate-active enzymes.Finally,this dissertation provides some theoretical foundation to promote maize resistance breeding and disease management.

关 键 词：玉米 九州镰孢菌 生物信息学 分泌蛋白 

分 类 号：S435.131[农业科学—农业昆虫与害虫防治]