Polyploidy Induction and Morphological Characteristics in the Asiatic Hybrid Lily(Lilium spp.cultivar dazzling)  

作　　者：Xiang Yixun Chen Mingxuan Chen Jianguo Yu Zhenyu Zhang Jinzhu Yang Tao Che Daidi Fan Jinping 

机构地区：[1]College of Horticulture and Landscape Architecture,Northeast Agricultural University,Harbin 150030,China [2]Key Laboratory of Cold Region Landscape Plants and Applications,Harbin 150030,China

出　　处：《Journal of Northeast Agricultural University(English Edition)》2024年第2期24-33,共10页东北农业大学学报（英文版）

基　　金：the China Postdoctoral Foundation(2023M730535)。

摘　　要：The Asiatic hybrid lily cultivar Lilium dazzling(family Liliaceae)is a perennial herbaceous bulbous plant with bright yellow flowers of ornamental values.It also shows resistance to diseases and abiotic stress,making it an ideal parent for breeding studies.This study established a sterile culture system using scales of dazzling lily as explants to induce polyploidy.Adventitious buds growing to 1 cm were treated with different concentrations of colchicine(125,250 and 500μmol·L-1),pendimethalin or trifluralin(100,200 and 300μmol·L-1,respectively)for 12,24 and 36 h.Flow cytometry and chromosome counting were used to identify ploidy,and the phenotype of the polyploids was examined by stomatal observation and leaf index determination.With increasing mutagen concentration and exposure time,the mortality and mutagenic rate increased.The optimal treatment group was:300μmol·L-1 trifluralin for 12 h,which resulted in 15.55%mortality rate and 42.22%induction rate.The polyploid plants showed a significant increase in stomata length,leaf length and leaf width,with a decrease in stomatal density compared with the control plants.This study provided a basis for polyploid breeding.

关 键 词：POLYPLOID Asiatic hybrid lily TRIFLURALIN flow cytometry plant morphology 

分 类 号：S436.8[农业科学—农业昆虫与害虫防治]