Analysis of Intraspecies Diversity of Rice Reveals Variation Patterns of Oryza sativa Basic Leucine Zippers  

作　　者：Jing Wang Wenqing Xiao Qingnan Wang Xu Wang Bin Jiang Yangchun Han Yang Sun 

机构地区：[1]Anhui Provincial Key Laboratory of Molecular Enzymology and Mechanism of Major Diseases,Key Laboratory for Conservation and Use of Important Biological Resources of Anhui Province,College of Life Sciences,Anhui Normal University,Wuhu,241000,China [2]Institute of Plant Protection,Jiangsu Academy of Agricultural Sciences,Nanjing,210000,China

出　　处：《Phyton-International Journal of Experimental Botany》2024年第5期859-873,共15页国际实验植物学杂志（英文）

基　　金：The National Natural Science Foundation of China(31871964,31801738,and 32100352);the Major Science and Technology Projects in Anhui Province(202003a06020009);Earmarked Fund for China Agriculture Research System(CARS-01-40);the Special Funds for Supporting Innovation and Entrepreneurship for Returned Oversea-Students in Anhui Province(2020LCX035);Anhui Provincial Natural Science Foundation(Youth Project,2008085QC148).

摘　　要：The basic leucine zipper(bZIP)is an important class of transcription factors in plants,playing a critical role in plant growth and development and responses to biotic and abiotic stress.Due to gene presence/absence variations,it is limited to identify bZIP genes based on the reference genome.Therefore,we performed the bZIP gene family analysis in the rice pan-genome.By employing a rice pan-genome,ninety-four OsbZIPs(72 core genes and 22 variable genes)were identified and divided into 11 groups in a phylogenetic tree.Based upon Ka/Ks values in 33 accessions,OsbZIPs were subjected to different selection pressures during domestication.The analysis of the effects of structural variations(SVs)on gene expression,gene structure,and conserved domains showed that SVs could significantly alter the expression levels of certain OsbZIPs,leading to gene truncation and the emergence of numerous atypical genes.Thirty-four differentially expressed OsbZIPs were identified by analyzing RNA-seq data of the Xanthomonas oryzae pv.oryzae(Xoo)infection susceptible(IR24)and resistant(IRBB67)lines under high temperature,and by counting the number of differentially expressed OsbZIPs in different subgroups.These Osb-ZIPs were found to respond to Xoo infection at an early stage and may not be involved in the mechanism of Xa4 and Xa7 resistance to Xoo.The multiple variation patterns of OsbZIP genes provide new insights into the OsbZIP genes in rice.These results provide new resources and offer new directions for functional studies of OsbZIPs.

关 键 词：RICE OsbZIP PAN-GENOME structure variation atypical gene bacterial blight 

分 类 号：S511[农业科学—作物学]