Genome-Wide Identification of Tomato (Solanum lycopersicum L.) CKX Gene Family and Expression Analysis in the Callus Tissue under Zeatin Treatment  

作　　者：Zhengfeng Lai Dongmei Lian Shaoping Zhang Yudong Ju Bizhen Lin Yunfa Yao Songhai Wu Jianji Hong Zhou Li 

机构地区：[1]Subtropical Agriculture Research Institute,Fujian Academy of Agricultural Sciences,Zhangzhou,363005,China

出　　处：《Phyton-International Journal of Experimental Botany》2024年第6期1143-1158,共16页国际实验植物学杂志（英文）

基　　金：funded by the Special Project for Science and Technology Innovation Platform of Fujian Academy of Agricultural Sciences,China(CXPT2023003);the Freely Explore Scientific and Technology Innovation Program of Fujian Academy of Agricultural Sciences(ZYTS202207);the Program for Innovative Research Team of Fujian Academy of Agricultural Sciences,China(CXTD2021006-3)。

摘　　要：The cytokinin oxidase/dehydrogenase(CKX)enzyme is essential for controlling thefluctuating levels of endogen-ous cytokinin(CK)and has a significant impact on different aspects of plant growth and development.Nonethe-less,there is limited knowledge about CKX genes in tomato(Solanum lycopersicum L.).Here we performed genome-wide identification and analysis of nine SlCKX family members in tomatoes using bioinformatics tools.The results revealed that nine SlCKX genes were unevenly distributed onfive chromosomes(Chr.1,Chr.4,Chr.8,Chr.10,and Chr.12).The amino acid length,isoelectric points,and molecular weight of the nine SlCKX proteins ranged from 453 to 553,5.77 to 8.59,and 51.661 to 62.494 kD,respectively.Subcellular localization analysis indi-cated that SlCKX2 proteins were located in both the vacuole and cytoplasmic matrix;SlCKX3 and SlCKX5 pro-teins were located in the vacuole;and SlCKX1,4,6,7,8,and 9 proteins were located in the cytoplasmic matrix.Furthermore,we observed differences in the gene structures and phylogenetic relationships of SlCKX proteins among different members.SlCKX1-9 were positioned on two out of the three branches of the CKX phylogenetic tree in the multispecies phylogenetic tree construction,revealing their strong conservation within phylogenetic subgroups.Unique patterns of expression of CKX genes were noticed in callus cultures exposed to varying con-centrations of exogenous ZT,suggesting their roles in specific developmental and physiological functions in the regeneration system.These results may facilitate subsequent functional analysis of SlCKX genes and provide valu-able insights for establishing an efficient regeneration system for tomatoes.

关 键 词：TOMATO SlCKX gene family phylogenetic relationships TRANS-ZEATIN expression pattern 

分 类 号：Q94[生物学—植物学]