压电型机械门控离子通道组件1在大鼠压力性损伤中的作用机制  

Mechanism of Piezo-type mechanosensitive ion channel component 1 in rat pressure injury

作　　者：孙家琪 卞璐 史文涛 吴学潮 鲁晓杰 Sun Jiaqi;Bian Lu;Shi Wentao;Wu Xuechao;Lu Xiaojie(Department of Neurosurgery,Central Hospital Affiliated to Jiangnan University,Wuxi 214026,Jiangsu Province,China;Institute of Wuxi Neurosurgery,Wuxi 214026,Jiangsu Province,China)

机构地区：[1]江南大学附属中心医院神经外科,江苏省无锡市214026 [2]无锡市神经外科研究所,江苏省无锡市214026

出　　处：《中国组织工程研究》2025年第8期1578-1584,共7页Chinese Journal of Tissue Engineering Research

基　　金：南京市卫生科技发展专项资金项目(重点项目:ZKX21063),项目负责人:史文涛;南京市卫生科技发展专项资金项目(YKK19129),项目负责人:史文涛;国家自然科学基金面上项目(82072791),项目负责人:鲁晓杰。

摘　　要：背景:压力性损伤的发生机制复杂,哪些因素在其发生中起到核心作用,这些因素具体又是如何发生的尚不完全清楚。目的:探讨压电型机械门控离子通道组件1(piezo-type mechanosensitive ion channel component 1,PIEZO1)与压力性损伤发生的关系。方法:①细胞实验:采用不同浓度的PIEZO1激动剂Yoda1干预人永生化角质形成细胞(HaCaT),检测细胞活性、钙离子内流及PIEZO1、凋亡相关蛋白的表达。②动物实验:采用随机数字表法将12只SD大鼠随机分为4组,每组3只:对照组大鼠不进行任何处理,1,2,3 mm组分别采用厚度1,2,3 mm的磁铁在大鼠背部两侧压迫1 h,建立压力性损伤模型,造模后切除全部创面组织,分别进行苏木精-伊红、Masson、免疫荧光染色及Western blot检测。结果与结论:①细胞实验:活/死细胞染色结果显示,随着Yoda1浓度(0,2.5,5,10μmol/L)的升高,HaCaT细胞凋亡增加;随着Yoda1浓度(0,5,10μmol/L)的升高,HaCaT细胞钙离子内流增加,并且随着处理时间的延长,钙离子内流增多;Western blot检测结果显示,与对照组(0μmol/L Yoda1干预)比较,5,10μmol/L Yoda1干预可提升HaCaT细胞中BAX、TG2、PIEZO1蛋白的表达,降低Bcl-2蛋白的表达;②动物实验:苏木精-伊红与Masson染色结果显示,3个实验组压迫部位皮肤结构被破坏,皮下脂肪液化坏死,胶原蛋白稀疏且排列紊乱,并且随着磁铁厚度(压力)的增加,压迫部位皮肤组织结构破坏程度加重;免疫荧光染色与Western blot检测结果显示,与对照组比较,3个实验组大鼠BAX、TG2、Yap1与PIEZO1蛋白表达升高,Bcl-2蛋白表达降低,并且随着磁铁厚度(压力)的增加,相关蛋白表达值改变更加明显;③结果表明:皮肤受压会激活PIEZO1使钙离子大量内流,随着所受压力的不断增加,最终导致钙超载后的细胞凋亡。BACKGROUND:The mechanisms underlying the occurrence of pressure injuries are complex,and it is not entirely clear which factors play a central role in the development of pressure injuries and how these factors operate.OBJECTIVE:To investigate the relationship between Piezo-type mechanosensitive ion channel component 1(Piezo1)and the occurrence of pressure injuries.METHODS:(1)Cellular experiment:Human immortalized keratinocytes(HaCaT)were treated with Yoda1,a Piezo1 agonist,at different concentrations.Cell viability,calcium ion influx,Piezo1,and apoptosis-related protein expression were detected.(2)Animal experiment:Twelve Sprague-Dawley rats were randomly divided into a control group and three experimental groups,with three rats in each group.The control group was not subjected to pressure,while in the three experimental groups,magnets with a thickness of 1,2,and 3 mm were used to press on both sides of the rats’back for 1 hour,respectively,to establish the animal models of pressure injuries.After modeling,all traumatic tissues were excised and subjected to hematoxylin-eosin,Masson,immunofluorescence staining and western blot assay.RESULTS AND CONCLUSION:Cellular experiments:The results of live/dead cell staining showed that HaCaT cell apoptosis increased with the increase of Yoda1 concentration(0,2.5,5,and 10μmol/L),and calcium ion influx increased with the increase of Yoda1 concentration(0,5,and 10μmol/L),as well as with the prolongation of treatment time.Western blot assay results showed an increase in the expression of BAX,TG2,and PIEZO1 and a decrease in the expression of the expression of Bcl-2 protein in HaCaT cells in 5 and 10μmol/L Yoda1 groups compared with the control group(0μmol/L Yoda1).Animal experiments:The results of hematoxylin-eosin and Masson staining showed that the skin structure of the three experimental groups was damaged at the compression site,there was subcutaneous fat liquefaction and necrosis,and collagen was sparse and disorganized,and damage to the skin structure at the compres

关 键 词：压力性损伤 压电型机械门控离子通道组件1 PIEZO1 钙超载 HACAT细胞 

分 类 号：R459.9[医药卫生—治疗学] R364[医药卫生—临床医学] R446.6