瓦草皂苷Ⅵ/Ⅶ的抗肿瘤作用及对BGC-823细胞凋亡及细胞周期的影响  

The antitumor effect of SinocrassulosidesⅥ/Ⅶand its effect on apoptosis and cell cycle of BGC-823 cells

作　　者：江婵玲 吴家慧 赵立春 王学勇[3] JIANG Chanling;WU Jiahui;ZHAO Lichun;WANG Xueyong(College of Pharmacy,Guangxi University of Chinese Medicine,Nanning 530200,China)

机构地区：[1]广西中医药大学药学院,南宁530200 [2]广西壮瑶药工程技术研究中心 [3]北京中医药大学中药学院

出　　处：《环球中医药》2024年第6期1050-1054,共5页Global Traditional Chinese Medicine

基　　金：国家自然科学基金(81860691);国家“青年岐黄学者”人才项目(2020-7);广西自然科学基金(2020GXNSFAA238021)。

摘　　要：目的 研究瓦草皂苷Ⅵ/Ⅶ对HepG-2、BGC-823、HT-29肿瘤细胞株体外增殖的影响,并检测其对BGC-823胃癌细胞凋亡及细胞周期的影响。方法 采用不同浓度的瓦草皂苷Ⅵ/Ⅶ(80、40、20、10、5μg/mL)分别作用于HepG-2、BGC-823、HT-29细胞24、48、72小时后,使用细胞增殖毒性试验(MTT法)检测瓦草皂苷Ⅵ/Ⅶ对HepG-2、BGC-823、HT-29细胞增殖的影响。采用流式细胞术检测细胞周期和细胞凋亡。结果 MTT结果显示,与空白对照组相比,瓦草皂苷Ⅵ/Ⅶ可明显抑制HepG-2、BGC-823、HT-29细胞的增殖并具有浓度依赖性,其中BGC-823细胞株对瓦草皂苷Ⅵ/Ⅶ最为敏感。流式细胞术实验表明,与空白组相比,瓦草皂苷Ⅵ/Ⅶ将BGC-823细胞阻滞于S期、G2/M期,抑制细胞进入G0/G1期,并促进BGC-823细胞的凋亡。细胞凋亡率从1.5%上升至8.6%,差异有统计学意义(P<0.01)。结论 瓦草皂苷VI/VII对HepG-2、BGC-823、HT-29肿瘤细胞株均表现出一定的抑制作用,尤其是对BGC-823细胞的抑制作用,其诱导BGC-823细胞的凋亡机制可能与细胞周期阻滞有关。Objective To study the effect of SinocrassulosidesⅥ/Ⅶon the proliferation of HepG-2、BGC-823、HT-29 tumor cell lines,and to detect its effect on apoptosis and cell cycle of BGC-823 gastric cancer cell line.Methods HepG-2,BGC-823 and HT-29 cells were treated with different concentrations of SinocrasulosidesⅥ/Ⅶ(80,40,20,10,5μg/mL)for 24,48,72 h,and then cell proliferation was detected by MTT assay.The cell cycle and apoptosis rate of BGC-823 cell line were detected by flow cytometry.Results The MTT results showed that SinocrasulosidesⅥ/Ⅶcould significantly inhibit the proliferation of HepG-2,BGC-823 and HT-29 cells in a concentration-dependent manner compared with the control group,and BGC-823 cell line was the most effective against SinocrasulosidesⅥ/Ⅶ.Flow cytometry showed that SinocrasulosidesⅥ/Ⅶcould arrest BGC-823 cells in S phase,G2/M phase,inhibit G0/G1 phase and promote apoptosis of BGC-823 cells from 1.5%to 8.6%(P<0.01).Conclusion SinocrasulosidesⅥ/Ⅶcan inhibit the proliferation of HepG-2,BGC-823,HT-29 cells,and the mechanism of inducing apoptosis of BGC-823 cells may be related to cell cycle arrest.

关 键 词：瓦草 三萜皂苷 抗肿瘤活性 细胞凋亡 细胞周期 

分 类 号：R285.5[医药卫生—中药学]