除湿止痒合剂对湿热蕴结型特应性皮炎患者肠道菌群的影响  

Effect of Chushi Zhiyang Decoction on Intestinal Flora of Atopic Dermatitis Caused by Dampness Heat Syndrome

作　　者：徐晓倩 吉凯峰 郑玉婷 陆冰萱 闫泳桦 高尚璞 李晓睿 李燕娜 程塞渊 吴孙思 张香坡 肖青青 宋瑜 XU Xiaoqian;JI Kaifeng;ZHENG Yuting;LU Bingxuan;YAN Yonghua;GAO Shangpu;LI Xiaorui;LI Yanna;CHENG Saiyuan;WU Sunsi;ZHANG Xiangpo;XIAO Qingqing;SONG Yu(Department of Dermatology,Longhua Hospital Shanghai University of Traditional Chinese Medicine,Shanghai 200032,China)

机构地区：[1]上海市中医药大学附属龙华医院皮肤科,上海200032

出　　处：《皮肤科学通报》2024年第3期305-312,共8页Dermatology Bulletin

基　　金：上海申康医院发展中心“促进市级医院临床技能与临床创新能力三年行动计划项目”(SHDC2020CR3092B);上海市科学技术委员会“科技创新行动计划”医学创新研究专项项目(20Y21901500);上海市科学技术委员会“科技创新行动计划项目”(23Y31920300)。

摘　　要：目的探讨除湿止痒合剂对特应性皮炎(AD)患者肠道菌群的影响。方法选取2021年5月—2022年10月上海中医药大学附属龙华医院皮肤科收治的154例AD患者,其中79例为试验组,予口服除湿止痒合剂4周,75例为对照组,予口服左西替利嗪4周;试验组、对照组在治疗前后均各采集粪便样品1次。通过高通量基因测序法分析治疗前后肠道菌群变化。结果本研究基于16rsDNA技术发现对照组、试验组用药后肠道菌群α多样性、β多样性差异不显著;试验组用药后肠道多种优势益生菌丰度上升,致病菌丰度下降,LefSe分析结果显示,用药后试验组在科水平上,乳杆菌科(Lactobacillaceae)、丛毛单胞菌科(Comamondaceae)、伯克氏菌科(Burkholderiaceae)为优势菌,在属水平上,真杆菌属(Eubacterium hallii group)、普雷沃氏菌属(Alloprevotella)、消化梭菌属(Peptoclostridium)、贪噬菌属(Variovorax)为优势菌;与对照组相比,试验组以霍尔德曼氏菌属(Holdemanella)、Catenisphaera为优势菌。结论除湿止痒合剂对AD患者肠道菌群生物多样性影响不显著,但能提升有益菌丰度,降低致病菌丰度。Objective To detect the effect of Chushi Zhiyang decoction on intestinal flora in patients with atopic dermatitis(AD).Methods In total,154 instances of AD in our hospital from May 2021 to October 2022 were classified as 79 cases in the test group which were given Chushi Zhiyang decoction for 4 weeks,and 75 cases in the control group were given levocetirizine for 4 weeks.Fecal samples were collected from the cases of experimental group and the control group before and after treatment.The changes of intestinal flora were analyzed by high-throughput gene sequencing.Results Based on 16rsDNA,there is no significant difference inαdiversity andβdiversity of intestinal flora.However,the abundance of multiple advantageous intestinal probiotics increased after treatment with Chushi Zhiyang decoction,and the abundance of pathogenic bacteria decreased in the experimental group.The results of LefSe showed that at the family level,Lactobacillaceae,Comamondaceae and Burkholderiaceae were the dominant bacteria after treatment.And at the genus level,Eubacterium hallii group,Alloprevotella,Peptoclostridium and Variovorax were the dominant bacteria.Compared with the control group,Holdemanella and Catenisphaera were the dominant bacteria in experimental group.Conclusion After Chushi Zhiyang decoction treatment,there is no significant effect on intestinal flora biodiversity in AD,but Chushi Zhiyang decoction can increase the abundance of beneficial bacteria and reduce the abundance of pathogenic bacteria.

关 键 词：特应性皮炎 肠道菌群 皮-肠轴 除湿止痒合剂 

分 类 号：R758.2[医药卫生—皮肤病学与性病学] R285[医药卫生—临床医学]