肠道菌群与2型糖尿病关系的两样本孟德尔随机化研究  

Relationship between intestinal flora and type 2 diabetes:a two-sample Mendelian randomization study

作　　者：陶丽宇 陆灏[1] TAO Li-yu;LU Hao(Shuguang Hospital,Shanghai University of Traditional Chinese Medicine,Shanghai 200032,China)

机构地区：[1]上海中医药大学附属曙光医院,上海200032

出　　处：《现代预防医学》2024年第12期2152-2158,共7页Modern Preventive Medicine

基　　金：国家自然科学基金资助项目(82074381,81874434);上海市临床重点专科建设项目-中医内分泌(shslczdzk05401);上海市卫生健康委员会中医药科研项目(2024BJ019)。

摘　　要：目的 利用两样本孟德尔随机化法探讨肠道菌群与2型糖尿病之间的因果关联。方法 在公开的全基因组关联研究数据库中获取暴露因素肠道菌群及结局变量2型糖尿病的单核苷酸多态性(SNP)数据;采用MR-Egger、加权中位数法、逆方差加权法(IVW)、简单模型、加权模型5种回归模型进行MR分析,采用MR-Egger法和IVW法进行异质性分析,MR-Egger截距测试数据的多效性,留一法进行敏感性分析,评估结果的稳健性。结果 在东亚人群中共有7种肠道菌群与2型糖尿病相关,其中危险菌有:Eubacteriumnodatumgroup,RuminococcaceaeUCG010,LachnospiraceaeNK4A136group,Victivall,保护菌有:Enterorhabdus,Erysipelatoclostridium,Romboutsia;在欧洲人群中共有4种肠道菌群与2型糖尿病相关,危险菌有:Howardella,Eubacteriumruminantiumgroup,Eubacteriumventriosumgroup,保护菌有:FamilyXIIIAD3011group。结论 特定肠道菌群对2型糖尿病风险具有一定因果关联。Objective To explore the causal association between gut microbiota and type 2 diabetes mellitus(T2DM)using two-sample Mendelian randomization.Methods The data of single nucleotide polymorphisms(SNP)of gut microbiota as an exposure factor and type 2 diabetes mellitus as an outcome variable were obtained from the public genome-wide association study database.MR-Egger,weighted median method,inverse variance weighted method(IVW),simple model,and weighted model were used for MR Analysis.The MR-Egger method and IVW method were used for heterogeneity analysis.MR-Egger intercept was used to test the pleiotropy of the data,and the leave-one-out method was used for sensitivity analysis to evaluate the robustness of the results.Results A total of 7 gut microbiota were found to be associated with type 2 diabetes in East Asians,and the risk bacteria were Eubacteriumnodatumgroup,Ruminococcaceae UCG010,Lachnospiraceae NK4A136 group,Victivall,and protect bacteria were Enterorhabdus,Erysipelatoclostridium,Romboutsia.There were a total of four kinds of intestinal flora in the European population associated with type 2 diabetes,dangerous bacteria were Howardella,Eubacteriumruminantiumgroup,Eubacteriumventriosumgroup,and protect bacteria were FamilyXIIIAD3011group.Conclusion Specific gut microbiota may have causal effects on the risk of type 2 diabetes.

关 键 词：肠道菌群 2型糖尿病 孟德尔随机化 

分 类 号：R587.1[医药卫生—内分泌]