0~3岁特异性皮炎婴幼儿患者肠道菌群差异分析  

Differential analyses of gut microbiota in infant patients with atopic dermatitis aged 0-3 years old

作　　者：令狐晨曦 孟仙[2] 陈静 查碧晴 邓思思 卢雯静 王红 王国庆 LINGHU Chen-xi;MENG Xian;CHEN Jing;ZHA Bi-qing;DENG Si-si;LU Wen-jing;WANG Hong;WANG Guo-qing(West China School of Public Health and West China Fourth Hospital,Sichuan University,Chengdu,Sichuan 610041,China;不详)

机构地区：[1]四川大学华西公共卫生学院/华西第四医院,四川成都610041 [2]四川省妇幼保健院 [3]成都东部新区公共卫生中心 [4]湖南省疾病预防控制中心

出　　处：《现代预防医学》2024年第12期2198-2203,2220,共7页Modern Preventive Medicine

基　　金：四川省科技厅重点研发项目(2019YFS0304)。

摘　　要：目的 通过病例对照研究探讨0~3岁特异性皮炎(Atopic dermatitis, AD)婴幼儿与健康婴幼儿肠道菌群的差异。方法 收集0~3岁AD患儿(AD组)与健康婴幼儿(HC组)的一般资料与粪便样品,采用统计学方法t检验和χ^(2)检验分析两组婴幼儿的一般资料,16S rRNA测序技术联合生物信息学分析比较两组肠道菌群的多样性和差异。结果 一般资料仅有直系亲属过敏史在两组间有显著差异(χ^(2)=13.875,P<0.001)。两组肠道菌群多样性差异无统计学意义;在门水平上,AD组疣微菌门相对丰度显著低于HC组(P=0.027),而梭杆菌门相对丰度显著高于HC组(P=0.038);在属水平上,AD组鲸杆菌属(P=0.010)、异常单胞菌属(P=0.013)、梭杆菌属(P=0.044)等菌属相对丰度显著高于HC组,而放线菌属(P=0.029)、柯林斯氏菌属(P=0.017)、埃格特菌属(P=0.028)等菌属相对丰度显著低于HC组。LEfSe分析结果显示AD组红蝽菌科(P=0.017)和长双歧杆菌(P=0.002)的相对丰度显著低于HC组。结论 AD组和HC组肠道菌群组成存在一定的差异,梭杆菌属、埃格特菌属、放线菌属和长双歧杆菌等差异微生物可能与AD的发生发展具有相关性,提示在生命早期调节肠道菌群可能预防及治疗AD。Objective To investigate the differences in gut flora between infants and children aged O~3 years with atopic dermatitis(AD)and healthy infants and children.Methods General information and fecal samples were collected from children with AD aged O-3 years(AD group)and healthy infants and children(HC group).T Test and Chi-Square Tests were used to analyze the general information of infants and children in two groups,and 16S rRNA sequencing technology combined with bioinformatics analysis to compare the diversity and differences of intestinal flora in two groups.Results General information only history of allergy in the immediate family was significantly different between the two groups(χ^(2)=13.875,P<0.001).The difference in the diversity of Gut microbiota between the two groups was not statistically significant.At the gate level,the relative abundance of Verrucomicrobiota was significantly lower in the AD group than in the HC group(P=0.027),whereas the relative abundance of Fusobacteriota was significantly higher than in the HC group(P=0.038).At the genus level,the relative abundance of the genera Cetobacterium(P=0.010),Dysgonomonas(P=0.013),and Fusicatenibacter(P=0.044)in the AD group was significantly higher than that in the HC group,while the relative abundance of the genera Actinomyces(P=0.029),Collinsella(P=0.017)and Eggerthella(P=0.028)were significantly lower in relative abundance than the HC group.LEfSe analysis showed that the relative abundance of Coriobacteriaceae(P=0.O17)and Bifidobacterium_longum(P=0.002)in the AD group was significantly lower than that in the HC group.Conclusion There are some differences in the composition of the Gut microbiota between the AD and HC groups.Differential microorganisms such as Fusicatenibacter,Eggerthella,Actinomyces and Bifidobacterium_longum may have a correlation with AD development,suggesting that regulating the Gut microbiota early in life may prevent and treat AD.

关 键 词：特异性皮炎 肠道菌群 16S rRNA测序 

分 类 号：R174.1[医药卫生—妇幼卫生保健]