化妆品中阿昔洛韦等6种抗病毒类药物的检测  

Detection of Six Antiviral Drugs Including Acyclovir in Cosmetics

作　　者：黎莉莉 陈伟浩 李永斌 谭志艺 Li Lili;Chen Weihao;Li Yongbin;Tan Zhiyi(Yunfu Institute for Food and Drug Control,Yunfu 527300,China;College of Pharmacy,Guangdong Medical University,Dongguan 523429,China)

机构地区：[1]云浮市食品药品检验所,广东云浮527300 [2]广东医科大学药学院,广东东莞523429

出　　处：《山东化工》2024年第11期168-171,188,共5页Shandong Chemical Industry

摘　　要：建立了化妆品中阿昔洛韦、更昔洛韦、喷昔洛韦、阿糖腺苷、利巴韦林和拉米夫定6种抗病毒类药物的高效液相色谱(HPLC)检测方法。采用十八烷基硅烷键合硅胶色谱柱,以甲醇-水为流动相,柱温:35℃,流速:1.0 mL·min^(-1),进样量:10μL,检测波长为207和273 nm进行梯度洗脱。阿昔洛韦等6种组分在0.2~50.0μg·mL^(-1)质量浓度范围内的线性关系良好,相关系数R^(2)均大于0.999,各加标水平浓度的平均回收率为86.128%~118.850%,相对偏差小于10%,日内精密度在0.090%~2.283%,日间精密度在0.058%~2.367%,方法的重复性与稳定性良好。该方法灵敏、准确、快速,阿昔洛韦等6种组分的检验参数均能满足方法需要,适合日后方法的推广与应用。This article established a high-performance liquid chromatography(HPLC)detection method for six antiviral drugs in cosmetics,including acyclovir,ganciclovir,penciclovir,adenosine arabinose,ribavirin,and lamivudine.It used octadecylsilane bonded silica gel chromatography column,methanol water as mobile phase,column temperature of 35℃,flow rate of 1.0 mL·min^(-1),and injection volume of 10μL.Gradient elution was performed with detection wavelengths of 207 and 273 nm.The linear relationship within the concentration range of six components including acyclovir at 0.2~50.0μg·mL^(-1) was good.The correlation coefficient R^(2) were all greater than 0.999.The average recovery rate of each spiked level concentration is 86.128%~118.850%,with a relative deviation of less than 10%.The intra day precision is 0.090%~2.283%,and the inter day precision is 0.058%~2.367%.The repeatability and stability of the method are good.This method is sensitive,accurate,and fast.The testing parameters for six components,including acyclovir,can meet the requirements of the method and are suitable for the promotion and application of the method in the future.

关 键 词：化妆品 高效液相色谱法 阿昔洛韦 更昔洛韦 喷昔洛韦 阿糖腺苷 利巴韦林 拉米夫定 

分 类 号：TQ658[化学工程—精细化工] O657.7[理学—分析化学]