鸡胚绒毛尿囊膜试验(HET-CAM)评价化妆品原料舒缓功效的创新方法建立和应用  

Establishment and Application of HET-CAM to Evaluate Soothing Effect of Cosmetic Ingredients

作　　者：刘慧 张绚 陈博文 刘菲 樊枚汝 杨素珍 Liu Hui;Zhang Xuan;Chen Bowen;Liu Fei;Fan Meiru;Yang Suzhen(Shandong Freda Biotech Co.,Ltd.,Jinan 250101,China)

机构地区：[1]山东福瑞达生物股份有限公司,山东济南250101

出　　处：《山东化工》2024年第11期207-210,215,共5页Shandong Chemical Industry

摘　　要：通过对经典的鸡胚绒毛尿囊膜试验(HET-CAM)进行创新改良,并对其评估化妆品原料的舒缓功效方面进行了初步应用。将0.05%浓度的SDS溶液滴加到鸡胚绒毛尿囊膜(CAM)表面,建立SDS刺激模型,作为阳性对照。将SDS溶液分别与不同浓度的舒缓原料配置成受试溶液,滴加到CAM表面,记录5 min内CAM开始出现出血、血管溶解、凝血的时间,通过计算IS值,评价受试溶液的刺激性。结果表明,0.05%的SDS能引起CAM的中度刺激性,而不同舒缓原料能够不同程度地减轻或完全拮抗0.05%SDS的刺激性。因此,本研究建立了CAM试验评价原料舒缓功效的方法,通过比较IS值大小,可判断不同原料拮抗SDS刺激性的程度,从而对原料的舒缓功效进行评价。A method was developed for evaluating the soothing effect of cosmetic ingredients by making innovative improvements to the classic HET-CAM,and the soothing effects of five cosmetic ingredients were studied.In this method,0.05%SDS solution was dripped onto the surface of the CAM to establish the SDS irritation model.The test solution was prepared by configuring the SDS solution with different concentrations of soothing cosmetics ingredients,which was then dropped onto the surface of the CAM.Within 5 minutes,the time of bleeding,vascular dissolution,and coagulation occurrence was recorded,and the IS value was calculated to evaluate the irritation of the tested solution.The results showed that 0.05%SDS can cause moderate irritation of the CAM,and different soothing ingredients can reduce or completely antagonize the irritation of 0.05%SDS.Therefore,a method is established to evaluate the soothing effect of ingredients by HET-CAM.By comparing the IS value,the degree of antagonism against SDS irritation of different ingredients is determined,which can be used to evaluate the soothing effect of ingredients.

关 键 词：鸡胚绒毛尿囊膜试验 SDS刺激模型 舒缓 

分 类 号：TQ658[化学工程—精细化工]