新型鹅细小病毒徐州分离株(NGPV XZ-01株)对半番鸭生长发育的影响  

Effects of Xuzhou strain of a Novel goose parvovirus(NGPV XZ-01 strain)on the growth and development of mule ducks

作　　者：迟兰[1] 蔺辉星[2] CHI Lan;LIN Huixing(Xuzhou Vocational College of Bioengineering,Xuzhou 221006,China;College of Veterinary Medicine,Nanjing Agricultural University,Nanjin 210095,China)

机构地区：[1]徐州生物工程职业技术学院,江苏徐州221006 [2]南京农业大学动物医学院,南京210095

出　　处：《黑龙江畜牧兽医》2024年第12期64-68,126-128,共8页Heilongjiang Animal Science And veterinary Medicine

基　　金：江苏省教育厅2022年江苏高校“青蓝工程”优秀青年骨干教师培养项目([2022]29);徐州市2021年现代农业面上项目(KC21144)。

摘　　要：为了探究新型鹅细小病毒徐州分离株(NGPV XZ-01株)对半番鸭生长发育的影响,试验将180只健康非免疫半番鸭随机分为3组,分别为对照组、3日龄攻毒组和7日龄攻毒组,每组60只,对照组于3日龄时皮下注射灭菌生理盐水0.2 mL,3日龄攻毒组和7日龄攻毒组分别于3日龄和7日龄时皮下注射NGPV XZ-01株病毒液(第7代,效价为5.7 lgELD_(50)/mL)0.2 mL,攻毒后观察并记录各组的生长发育情况、临床症状、发病和死亡情况,并对喙长和体重进行测定,于25日龄时全部扑杀,进行剖检、病理切片观察和X光检查。结果表明:攻毒后,3日龄攻毒组和7日龄攻毒组均生长发育迟缓或成僵鸭;24日龄时,3日龄攻毒组和7日龄攻毒组的体型明显小于对照组,3日龄攻毒组体型明显小于7日龄攻毒组。试验期间,3日龄攻毒组和7日龄攻毒组表现为精神萎靡不振、喜卧、打堆、排白色或黄白色粪便,部分患鸭出现运动障碍、瘫痪等症状;随着日龄的增长,上下喙变短及舌头外露的症状逐渐明显。其中3日龄攻毒组发病率为90.0%、死亡率为11.7%;7日龄攻毒组发病率为76.7%、死亡率为6.7%;对照组无发病和死亡情况。10~22日龄时,3日龄攻毒组和7日龄攻毒组的体重和喙长均显著低于对照组(P<0.05),3日龄攻毒组的体重和喙长均显著低于7日龄攻毒组(P<0.05)。3日龄攻毒组和7日龄攻毒组剖检可见泄殖腔膨大,肠道内容物稀薄,肝脏均出现水肿、质脆等病变;病理切片观察可见十二指肠肠绒毛顶端上皮细胞坏死,十二指肠绒毛不同程度脱落,部分肠腺坏死;部分肝细胞出现坏死,肝窦淤血,有炎性细胞浸润;其他脏器未见明显病变。对照组未见明显异常。X光检查可见3日龄攻毒组和7日龄攻毒组下喙、翅、腿等部位明显小于对照组,且胫骨长度远低于对照组。说明NGPV XZ-01株可使半番鸭的生长发育明显受阻,具有很强的致病性,且对半番鸭的影响随In order to investigate the effects of Xuzhou strain of a Novel goose parvovirus(NGPV XZ-01 strain)on the growth and development of mule ducks,in the experiment,180 healthy non-immunized mule ducks were randomly divided into three groups,namely the control group,the 3-day-old challenge group and the 7-day-old challenge group,and each group had 60 animals.In the control group,0.2 mL of sterilized saline was injected subcutaneously at 3 days of age;in the 3-day-old challenge group and 7-day-old challenge group,0.2 mL of virus solution of NGPV XZ-01 strain(7th generation,titer 5.7 lgELD_(50)/mL)was injected subcutaneously at 3 days of age and 7 days of age,respectively.After challenge,the growth and development,clinical symptoms,morbidity and mortality of each group were observed and recorded;beak length and body weight were measured;all ducks were killed at 25 days of age and subjected to autopsy,pathologic section observation and X-ray examination.The results showed that the 3-day-old and 7-day-old challenge groups were stunted or became stiff ducks after challenge;at 24 days of age,the body sizes of the 3-day-old and 7-day-old challenge groups were significantly smaller than that of the control group,and the body size of the 3-day-old group was significantly smaller than that of the 7-day-old challenge group.During the test period,the 3-day-old and 7-day-old challenge groups showed depressed spirit,lying,piling up,and white or yellowish-white feces;some ducks showed symptoms such as movement disorders and paralysis.With the increase of age,the symptoms of short upper and lower beak and exposed tongue became obvious.The morbidity rate was 90.0%and mortality rate was 11.7%in the 3-day-old challenge group,76.7%and 6.7%each in the 7-day-old challenge group,and there was no morbidity and mortality in the control group.From 10 to 22 days of age,the body weight and beak length of the 3-day-old challenge group and the 7-day-old challenge group were significantly lower than those of the control group(P<0.05);the body weig

关 键 词：鸭短喙侏儒综合征 新型鹅细小病毒 徐州分离株 半番鸭 生长发育 

分 类 号：S855.3[农业科学—临床兽医学]