茶树精油纳米乳剂的制备及其质量评价  

Preparation of tea tree oil nanoemulsion and its quality evaluation

作　　者：田颖 唐歆 张宏伟 王天宇 吴晓雯 陈洪博[2] 曹翀 张凯龙 邱龙新[2] TIAN Ying;TANG Xin;ZHANG Hongwei;WANG Tianyu;WU Xiaowen;CHENG Hongbo;CAO Chong;ZHANG Kailong;QIU Longxin(College of Animal Science(College of Bee Science),Fujian Agriculture and Forestry University,Fuzhou 350002,China;Fujian Provincial Key Laboratory of Preventive Veterinary Medicine and Biotechnology,College of Life Science,Longyan University,Longyan 360012,China)

机构地区：[1]福建农林大学动物科学学院(蜂学学院),福州350002 [2]龙岩学院生命科学学院预防兽医学与生物技术福建省高等学校重点实验室,福建龙岩360012

出　　处：《黑龙江畜牧兽医》2024年第12期94-100,129,共8页Heilongjiang Animal Science And veterinary Medicine

基　　金：福建省科技创新重点项目(2021G02015);福建省科技计划高校产学合作项目(2023N5009);新罗区产学研科技联合创新项目(2022XLXYZ007);新罗区奇迈科技创新基金项目(XLQM008)。

摘　　要：为了制备稳定性高的茶树精油(tea tree oil, TTO)纳米乳剂,并评价制备的TTO纳米乳剂的质量,试验采用高能剪切法制备TTO纳米乳剂,根据伪三元相图确定TTO纳米乳剂的最优配方。对采用最优配方制备的TTO纳米乳剂进行外观性状观察,采用染色法进行类型鉴定,使用pH计测定pH值,通过透射电子显微镜观察表面形貌,使用激光粒度仪测定粒径和电位,并考察其稳定性,利用二倍稀释法测定TTO纳米乳剂和TTO对巴氏杆菌和沙门氏菌的最低抑菌浓度(MIC)。结果表明:TTO纳米乳剂的最优配方为12.95%蓖麻油聚氧乙烯醚(EL-40)、6.5%聚乙二醇400(PEG-400)、8.33%TTO、72.22%纯化水。采用最优配方制备的TTO纳米乳剂为水包油(O/W)型乳剂,平均pH值为8.32,平均粒径为15.7 nm,表面电位为(-4.29±0.26)mV。将TTO纳米乳剂加水按1∶20~1∶120稀释,静置24 h后观察无分层或沉淀现象;4 000,6 000,8 000,10 000 r/min分别离心20 min后,纳米乳剂的外观性状无变化,也无沉淀、分层等现象产生。在不同温度(-20℃、4℃、25℃、40℃、60℃、80℃)下处理30 min后,其外观无明显变化,且60℃加热后其外观更透明,80℃加热后出现分层现象;在4℃下放置30 d后观察,无任何异常变化。用pH值为5,6,7,8,9的水溶液将TTO纳米乳剂分别按1∶100、1∶1 000、1∶10 000稀释后静置24 h观察,无沉淀或絮状物等产生。TTO纳米乳剂对巴氏杆菌和沙门氏菌的MIC值分别为39.65 mg/mL、29.74 mg/mL,优于未纳米乳化的TTO(对巴氏杆菌和沙门氏菌的MIC值均为49.57 mg/mL)。说明TTO纳米乳剂能够解决TTO易挥发、难溶于水等问题,制备的TTO纳米乳剂具有纳米级粒径且尺寸均一,稳定性强,易于储存,易溶于水,具有良好的抑菌性。In order to prepare tea tree oil(TTO)nanoemulsion with high stability,and evaluate the quality of the prepared TTO nanoemulsion,in the experiment,TTO nanoemulsion was prepared by high-energy shear method,and the optimal formulation of TTO nanoemulsion was determined based on the pseudo-ternary phase diagram.Appearance and characterization of TTO nanoemulsion were observed by optimal formulation.Type identification was carried out by staining method and pH was determined using pH meter.The surface morphology was observed by transmission electron microscopy,and the particle size and potential were determined and examined for stability by a laser particle sizer;the minimum inhibitory concentration(MIC)of TTO nanoemulsion and TTO against Pasteurella and Salmonella.was determined by using the two fold dilution method.The results showed that the optimal formulation of TTO nanoemulsion was 12.95%castor oil polyoxyethylene ether(EL-40),6.5%polyethylene glycol 400(PEG-400),8.33%TTO,and 72.22%purified water.The TTO nanoemulsion prepared by using the optimal formulation was an oil-in-water(O/W)type emulsion with an average pH value of 8.32,an average particle size of 15.7 nm and a surface potential of(-4.29±0.26)mV.The TTO nanoemulsion was diluted 20-120 times with water and left to stand for 24 h without any delamination or precipitation.After centrifugation at 4000,6000,8000,10000 r/min for 20 min,there was no change in the appearance and properties of the nanoemulsion,and there was no precipitation,delamination and other phenomena produced.After being treated at different temperatures(-20℃,4℃,25℃,40℃,60℃,80℃)for 30 min,there was no obvious change in its appearance,and its appearance was more transparent after being heated at 60℃,and delamination appeared after being heated at 80℃.It was observed after 30 d at 4℃without any abnormal changes.The TTO nanoemulsion was diluted 100 times,1000 times and 10000 times with aqueous solutions of pH value 5,6,7,8 and 9,respectively,and then observed after 24 h of s

关 键 词：茶树精油 纳米乳剂 制备 质量评价 抑菌试验 

分 类 号：S859[农业科学—临床兽医学]