非共轭聚合物点和Eu^(3+)的比率荧光传感器检测牛奶中的四环素  

Detection of tetracycline in milk by ratio fluorescence sensor based on non-conjugated polymer dots and Eu^(3+)

作　　者：尹欣越 邓心怡 相玉红[1] 叶能胜[1] YIN Xinyue;DENG Xinyi;XIANG Yuhong;YE Nengsheng(Department of Chemistry,Capital Normal University,Beijing 100048)

机构地区：[1]首都师范大学化学系,北京100048

出　　处：《首都师范大学学报（自然科学版）》2024年第3期97-107,共11页Journal of Capital Normal University：Natural Science Edition

基　　金：北京市教育委员会科研计划项目(KM202010028006)。

摘　　要：四环素(TC)因其广谱抗菌活性和低廉的价格被广泛应用于畜牧养殖业治疗及预防相关细菌感染,因其难以代谢而残留在动物源性食品中,影响人类健康,因此建立方便快捷的TC检测方法至关重要。本文以高支化聚乙烯亚胺(PEI)和抗坏血酸(AA)为原料,合成了一种水溶性良好且具有高稳定性蓝绿色荧光的非共轭聚合物点(NCPDs),其通过与Eu^(3+)和柠檬酸三钠的简单混合构建了检测TC含量的比率荧光探针。TC既可以通过内滤效应淬灭NCPDs在490 nm处的蓝绿色荧光,也可以通过Eu^(3+)的天线效应增敏Eu^(3+)在618 nm处的红色荧光。二者荧光强度的比值与TC的含量具有良好的线性关系。该方法具有快速的荧光响应,较宽的线性范围50.0 nmol/L~80.0μmol/L和较低的检出限11.8 nmol/L,并且该比率荧光传感器成功地测定了牛奶样品中的TC。In order to treat and prevent related bacterial infections,tetracycline(TC)is widely used in animal husbandry and breeding industry due to its broad-spectrum antibacterial activity and low price.Because TC is difficult to be metabolized and its residues in animal derived foods cause harm to human health.Therefore,it is crucial to establish a convenient and fast detection method for TC.In this work,a non-conjugated polymer dots(NCPDs)with good water solubility and high stability blue-green fluorescence were synthesized based on high branched polyethylene imine(PEI)and ascorbic acid(AA).The ratio fluorescence probe for detecting TC content was constructed by simple mixing NCPDs with Eu^(3+)and sodium citrate dihydrate.TC can quench the blue-green fluorescence of NCPDs at 490 nm through internal filtering effect,and can also sensitize the red fluorescence of Eu^(3+)at 618 nm through the antenna effect of Eu^(3+).The ratio of fluorescence intensity of 618 and 490 nm has a good linear relationship with the concentration of TC.The method has a rapid fluorescence response with a wider linear range of 50.0 nmol/L to 80.00μmol/L and a lower limit of detection of 11.8 nmol/L,and the ratiometric fluorescence sensor successfully determined the TC in the milk sample.

关 键 词：非共轭聚合物点 比率荧光传感器 天线效应 内滤效应 四环素 

分 类 号：O65[理学—分析化学]