蕲艾精油纳米乳的制备及伪三元相图的绘制  

Preparation and Pseudo Ternary Phase Diagram Plotting Method of Nanoemulsion Containing Artemisia Argyi Essential Oil

作　　者：卢博成 程丽媛 彭小航 丁洁琼[2] LU Bo-cheng;CHENG Li-yuan;DING Jie-qiong(School of Pharmacy,Xianning Medical College,Hubei University of Science and Technology,Xianning Hubei 437100,China)

机构地区：[1]湖北科技学院医学部药学院,湖北咸宁437100 [2]湖北科技学院医学部基础医学院

出　　处：《湖北科技学院学报（医学版）》2024年第4期297-300,共4页Journal of Hubei University of Science and Technology(Medical Sciences)

摘　　要：目的 制备蕲艾精油纳米乳,提高艾叶精油稳定性,解决艾叶精油易挥发、易变质、不易长期保存的问题。方法 采用机械法制备蕲艾叶精油纳米乳,通过离心实验筛选乳化剂泊洛沙姆188、吐温20、吐温80,助乳化剂无水乙醇、甘油、正丁醇,以及油水相的比例,制备的乳剂通过粒径分析仪、光学显微镜对粒径、PDI、ζ电位、形态表征。采用含水量-电导率法绘制蕲艾精油纳米乳伪三元相图,确定最佳处方工艺。结果 经筛选制备的蕲艾精油纳米乳最佳工艺为:乳化剂泊洛沙姆188、助乳化剂无水乙醇、油相大豆油、水相为去离子水、该乳剂的粒径91.1nm、ζ电位-6.63mv、PDI为0.225、Km值为0.375,高速离心后未见沉淀以及油水分离。结论 用该方法制备的蕲艾精油纳米乳制备工艺简单,稳定性好,为进一步推广蕲艾精油打下基础。Objective To prepare the nanoemulsion of A.argyi essential oil,improve the stability of A.argyi essential oil,and solve the problem of volatile deterioration and difficulty to long term storage of A.argyi essential oil.Methods The mechanical method was used to prepare the nanoemulsion of essential oil of A.argyi leaves,and the emulsifiers Poloxamer188,Tween 20,Tween 80,the co-emulsifier anhydrous ethanol,glycerol,n-butanol and the proportion of oil-water phase were screened by centrifugation.The prepared emulsion was characterized by a particle size analyzer and optical microscope for particle size,PDI,ζ potential and morphology.The water content conductivity method was used to draw the pseudo-ternary phase diagram of A.argyi essential oil nanoemulsion to determine the optimal process.Results The optimal process for the preparation of A.argyi essential oil nanoemulsion were as follows:emulsifier Polysorbate 188,co-emulsifier anhydrous ethanol,oil phase soybean oil,aqueous phase deionized water,with a particle size of 91.1 nm,ζ potential of-6.63 mV,PDI of 0.225 and Km value of 0.375.After high-speed centrifugation,no-precipitation or phase separation was observed.Conclusion The nanoemulsion of A.argyi essential oil prepared in this experiment was easily available and stable,and laid a foundation for the further promotion of A.argyi essential oil.

关 键 词：载药体系 纳米乳 伪三元相图 电导率 Ζ电位 PDI 

分 类 号：R966[医药卫生—药理学]