基于PBNPs-Ag纳米粒子的ROS清除和抗菌协同作用促进伤口愈合行为研究  

Study on the ROS Scavenging and Antibacterial Synergistic Effect of PBNPs-Ag Nanoparticles for Wound Healing

作　　者：唐冬旭 刘坤 付娌丽 周士忠 张洋林 李月生[2,3] TANG Dong-xu;LIU Kun;LI Yue-sheng(School of Pharmacy,Xianning Medical College,Hubei University of Science and Technology,Xianning Hubei 437100,China)

机构地区：[1]湖北科技学院医学部药学院,湖北咸宁437100 [2]湖北科技学院辐射化学与功能材料湖北省重点实验室 [3]湖北科技学院核技术与化学生物学院

出　　处：《湖北科技学院学报（医学版）》2024年第4期301-305,F0002,共6页Journal of Hubei University of Science and Technology(Medical Sciences)

摘　　要：目的利用普鲁士蓝纳米颗粒(PBNPs)与纳米银粒子(Ag)制备一种兼具ROS清除与抗菌作用的PBNPs-Ag纳米粒子以用于促进伤口愈合。方法通过XRD、IR、Zeta电位、粒径测量对PBNPs-Ag进行表征。在细胞层面上,首先使用MTT法测试PBNPs-Ag的细胞毒性,接下来利用H_(2)O_(2)作为ROS引发剂,建立细胞ROS预防模型。利用DCFH-DA对细胞内ROS直接染色。最后,利用抑菌圈法测试PBNPs-Ag对细菌的抑制效果。结果PBNPs-Ag纳米粒子粒径均匀,结晶度高,无明显细胞毒性,具有良好的ROS清除能力与抗菌能力。结论PBNPs-Ag是一种效果良好,具有前景的治疗皮肤损伤的纳米颗粒药物。Objective To combine Prussian blue nanoparticles(PBNPs)and silver nanoparticles(Ag)to synthesize PBNPs-Ag nanoparticles with both ROS scavenging and antibacterial effects for promoting wound healing.Methods X-ray diffraction(XRD),infrared spectroscopy(IR),Zeta potential analysis,particle size measurements were employed to characterize the physicochemical properties of PBNPs-Ag.At the cellular level,the cytotoxicity of PBNPs-Ag was tested using the MTT assay followed by establishing a cellular model for ROS prevention utilizing H_2O_(2)as a ROS initiator.Additionally,DCFH-DA staining was performed for direct visualization of the intracellular ROS levels.Finally,an inhibition circle assay was conducted to evaluate the inhibitory effect of PBNPs-Ag on bacteria.Results The synthesized PBNPs-Ag nanoparticles exhibited uniform particle size distribution,high crystallinity and negligible cytotoxicity.Moreover,they demonstrated excellent capability in scavenging ROS and antibacterial abilities.Conclusion PBNPs-Ag is an effective and promising nanoparticle drug for the treatment of skin injuries.

关 键 词：普鲁士蓝 银纳米粒子 ROS清除 抗菌 皮肤损伤 

分 类 号：R318[医药卫生—生物医学工程]