肾衰营养胶囊对肾性骨病模型大鼠肾功能和骨代谢的改善作用及其对BMP-7/Smads信号通路的影响  

Improvement effect of Shenshuai Yingyang Capsule on renal function and bone metabolism in renal osteodystrophy model rats and its effect on BMP-7/Smads signaling pathway

作　　者：陈杰彬 胡蓉[2] 吕佩佳 李成杰 魏连波 CHEN Jiebin;HU Rong;LYU Peijia;LI Chengjie;WEI Lianbo(Department of Nephrology,Hospital of integrated Traditional Chinese and Western Medicine,Southern Medical University,Guangzhou 510315,China;Department of Traditional Chinese Internal Medicine,School of Traditional Chinese Medicine,Southern Medical University,Guangzhou 510515,China;Department of Traditional Chinese Medicine,Shenzhen Hospital,Southern Medical University,Shenzhen 518000,China)

机构地区：[1]南方医科大学中西医结合医院肾病科,广东广州510315 [2]南方医科大学中医药学院中医内科,广东广州510515 [3]南方医科大学深圳医院中医科,广东深圳518000

出　　处：《吉林大学学报（医学版）》2024年第3期658-665,共8页Journal of Jilin University：Medicine Edition

基　　金：国家自然科学基金青年科学基金项目(82104795)。

摘　　要：目的:探讨肾衰营养胶囊对肾性骨病模型大鼠的改善作用及其对骨形态发生蛋白(BMP)-7/Smads信号通路的影响,阐明肾性骨病模型大鼠肾功能和骨代谢与肾性骨病的关系。方法:选取50只SPF级SD大鼠,随机选取10只大鼠作为对照组,另外40只大鼠建立肾性骨病模型。将30只造模成功大鼠分为模型组、阳性对照组和肾衰营养胶囊组,每组各10只。对照组和模型组大鼠采用生理盐水灌胃,阳性对照组大鼠给予0.01 mg·kg^(-1)骨化三醇灌服,肾衰营养胶囊组大鼠给予1.2 g·kg^(-1)肾衰营养胶囊灌服,均灌胃12周。采用HE染色观察各组大鼠股骨组织病理形态表现,全自动生化分析仪和化学发光法检测各组大鼠血清中肾功能和钙磷代谢指标,实时荧光定量PCR(RT-qPCR)法检测各组大鼠股骨组织中BMP-7和Smad1/5/8 mRNA表达水平,Western blotting法检测各组大鼠股骨组织中BMP-7、磷酸化BMP-7(p-BMP-7)、Smad1/5/8和磷酸化Smad1/5/8(p-Smad1/5/8)蛋白表达水平。结果:HE染色,对照组大鼠骨小梁排列正常,成骨细胞和类骨质面积未发生改变;模型组大鼠骨小梁宽度和平均类骨质面积增加,成骨细胞数量减少;阳性对照组和肾衰营养胶囊组大鼠骨小梁宽度及平均类骨质面积减小,成骨细胞数量增加。与对照组比较,模型组大鼠血尿素氮(BUN)和血肌酐(Scr)水平均升高(P<0.05);与模型组比较,阳性对照组和肾衰营养胶囊组大鼠BUN及Scr水平均降低(P<0.05);与阳性对照组比较,肾衰营养胶囊组大鼠BUN和Scr水平均降低(P<0.05)。与对照组比较,模型组大鼠血清中钙离子(Ca2+)水平降低(P<0.05),磷离子(P3-)、甲状旁腺激素(PTH)和碱性磷酸酶(ALP)水平均升高(P<0.05);与模型组比较,阳性对照组和肾衰营养胶囊组大鼠血清中Ca2+水平升高(P<0.05),P3-、PTH和ALP水平均降低(P<0.05);与阳性对照组比较,肾衰营养胶囊组大鼠血清中Ca2+水平升高(P<0.05),P3-、PTH和ALP水平均降低Objective:To discuss the improvement effect of Shenshuai Yingyang Capsule in the renal osteodystrophy model rats and its effect on the bone morphogenetic protein(BMP)-7/Smads signaling pathway,and to clarify the relationship between renal function and bone metabolism in the renal osteodystrophy model rats.Methods:Fifty SPF SD rats were selected,and 10 of which were randomly chosen as control group,and the remaining 40 rats were used to establish the renal osteodystrophy model.Thirty successful modeling rats were divided into model group,positive control group,and Shenshuai Yingyang Capsule group,and there were 10 rats in various groups.The rats in control and model groups were given saline,the rats in positive control group were given 0.01 mg·kg~(-1) calcitriol,and the rats in Shenshuai Yingyang Capsule group were given 1.2 g·kg~(-1) Shenshuai Yingyang Capsule,all the rats were treated for 12 weeks.HE staining was used to observe the pathomorphology of femur tissue of the rats in various groups;automatic biochemical analyzer and chemiluminescence were used to detect the kidney function and calcium-phosphorus metabolism indicators in serum of the rats in various groups;realtime fluorescence quantitative PCR(RT-qPCR) method was used to detect the expression levels of BMP-7 and Smad1/5/8 mRNA in femur tissue of the rats in various groups;Western blotting method was used to detect the expression levels of BMP-7,phosphorylated BMP-7(p-BMP-7),Smad1/5/8,and phosphorylated-Smad1/5/8(p-Smad1/5/8) proteins in femur tissue of the rats in various groups.Results:The HE staining results showed that there was normal trabecular arrangement in control group with unchanged osteoblasts and osteoid area.In model group,the trabecular width and average osteoid area were increased,while the number of osteoblasts was decreased.In positive control and Shenshuai Yingyang Capsule groups,the trabecular width and average osteoid area were decreased,and the number of osteoblasts was increased.Compared with control group,the levels of blood

关 键 词：肾性骨病 肾衰营养胶囊 骨形态发生蛋白7 Smad同源物重组蛋白 骨密度 

分 类 号：R692[医药卫生—泌尿科学] R681[医药卫生—外科学]